Abstract
The morphogenic capacity of Digitalis obscura leaf explants cultured in vitro has been studied, noting factors promoting the differentiation of roots, buds and shoots as well as those promoting callus proliferation. Complete plant regeneration was obtained only by first culturing the leaf explants in a medium with NAA and BA to induce formation of buds, and subsequently transferring them to a medium without growth regulators to achieve the further development of shoots.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indoleacetic acid
- NAA:
-
naphthaleneacetic acid
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Communicated by M. H. Zenk
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Pérez-Bermúdez, P., Brisa, M.C., Cornejo, M.J. et al. In vitro morphogenesis from excised leaf explants of Digitalis obscura L.. Plant Cell Reports 3, 8–9 (1984). https://doi.org/10.1007/BF00270218
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DOI: https://doi.org/10.1007/BF00270218