Abstract
This report describes the development of procedures for the production of somatic embryos in cell cultures of Glycine species including soybean. The conditions for callus induction and initiation of rapidly growing cell suspension cultures were defined. Methods for inducing embryogenesis were tested on 16 lines of several Glycine species and cultivars of soybean. The SB-26 Culture of a G. soja gave the best results and was used in the experiments. Embryogenesis required the presence of picloram or 2,4-D. AMO 1618, CCC, PP-333 and Ancymidol enhanced the embryogenesis frequency. Plants of the G. soja (SB-26) were grown to maturity from seed-derived shoot tips. Characteristics of the plants are discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BA:
-
Benzyladenine
- NAA:
-
Naphthaleneacetic acid
- IAA:
-
Indoleacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA:
-
Gibberellic acid
- AMO:
-
1618, CCC, PP-333, MS, B5 (See text)
References
Cheng T, Saka H, Voqui-Ding TH (1980) Plant Sci Lett 19:91–99
Collins GB, Phillips GC (1982) in Variability in Plants Regenerated from Tissue Culture, Earle ED, Demarly Y (Eds) Praeger Scientific Publishers, New York pp 22–34
Coolbaugh RC, Hamilton R (1976) Plant Physiol 49:245–248
Gamborg OL (1975) in Plant Tissue Culture Methods Gamborg OL, Wetter, LR (Eds), National Res Council, Saskatoon Canada pp 1–10
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:150–158
Gamborg OL, Constabel F, Miller RA (1970) Planta 95:355–358
Kartha KK, Pahl K, Leung NL, Mroginski LA (1981) Can J Bot 59:1671–1679
Paleg L (1972) Plant Physiol 49:417
Phillips GC, Collins GB (1981) Plant Cell Tissue Organ Culture 1:123–129
Widholm JM, Rick S (1983) Plant Cell Reports 2:19–20
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gamborg, O.L., Davis, B.P. & Stahlhut, R.W. Somatic embryogenesis in cell cultures of Glycine species. Plant Cell Reports 2, 209–212 (1983). https://doi.org/10.1007/BF00270106
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270106