Abstract
Fine cell suspension cultures of Cinchona ledgeriana produce only very low amounts of quinoline alkaloids. These cultures formed self-propagating compact globular structures (CGS) on medium containing 2,4-D and BAP. These CGS could be induced to produce significant amounts of quinoline alkaloids by replacing 2,4-D by low amounts of 1-NAA, which was accompanied by histological changes of the CGS. A few high producing CGS clones could be selected. The stability of this trait was studied over a period of about one year of culture in maintenance medium.
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Abbreviations
- BAP:
-
Benzylaminopurine
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- 1-NAA:
-
1-Naphthylacetic acid
- CGS:
-
compact globular structures
References
Anderson LA, Keene AT, Phillipson JD(1982) Planta Med.46:25–27
Gamborg OL, Miller RA, Ojima K(1968) Expt. Cell Res. 50:151–158
Harkes PAA, Krijbolder L, Libbenga KR, Wijnsma R, Nsengiyeremge T, Verpoorte R(1985) Plant Cell Tissue and Organ Culture 4:199–214
Mulder-Krieger Th, Verpoorte R, Graaf YP de, Kreek M van der, Baerheim-Svendsen A(1982a)Planta Med. 46:15–18
Mulder-Krieger Th, Verpoorte R, Water A de, Gessel M van, Oeveren BCJA van, Baerheim-Svendsen A(1982b) Planta Med. 46:19–24
Robins RJ, Payne J, Rhodes MJC(1986) Planta Med.52:220–226
Rhodes MJC, Payne J, Robins RJ(1986) Plant Med.52:226–229
Scragg AH, Allan EJ, Morris P(1988) J. Plant Physiol. 132:184–189
Singer SR(1985a) Can. J. Botany 64:233–237
Singer SR(1985b) Can. J. Botany 64:238–241
Smith EJ(1984) J. Chromatography 299:233–244
Staba EJ, Chung AC(1981) Phytochemistry 20:2495–2498
Wijnsma R, Vliet TB van, Harkes PAA, Groningen HJ van, Heijden R van der, Verpoorte R, Baerheim-Svendsen A (1987) Planta Med.53:80–84
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Communicated by W. Barz
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Hoekstra, S.S., Harkes, P.A.A., Verpoorte, R. et al. Effect of auxin on cytodifferentiation and production of quinoline alkaloids in compact globular structures of Cinchona ledgeriana . Plant Cell Reports 8, 571–574 (1990). https://doi.org/10.1007/BF00270055
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DOI: https://doi.org/10.1007/BF00270055