Abstract
Various fluorescent compounds — carboxyfluorescein, scopoletin, fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), rhodamine 123, and rhodamine B ethyl ester — were used to study their effects on calcium-induced fusion of fusogenic carrot (Daucus carota L.) protoplasts. These protoplasts normally fused at a high percentage (50–60%) in response to 10 mM calcium, pH 6.0; however, if cells had been labeled with scopoletin, FITC, or RITC, fusion was greatly reduced. In contrast, labeling with carboxyfluorescein, rhodamine 123, or rhodamine B ethyl ester had no detectable effect on calcium-induced fusion. The two rhodamine dyes are shown to be localized in mitochondria.
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Abbreviations
- EGTA:
-
ethyleneglycol-bis-(β-aminoethyl ether) N′,N′-tetraacetic acid
- FITC:
-
fluorescein isothiocyanate
- RITC:
-
rhodamine isothiocyanate
- PE:
-
phosphatidylethanolamine
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Communicated by J. M. Widholm
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Kanchanapoom, K., Boss, W.F. The effect of fluorescent labeling on calcium-induced fusion of fusogenic carrot protoplasts. Plant Cell Reports 5, 252–255 (1986). https://doi.org/10.1007/BF00269814
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DOI: https://doi.org/10.1007/BF00269814