Summary
The storage protein content of somatic embryos of Gossypium hirsutum L. cv. Coker 201 was determined using extinction level, antigen/antibody association detection methods. Mature storage protein was first detected in early globular-stage somatic embryos at a total concentration of 0.36% of the embryo protein mass. Tulip-stage and mature somatic embryos were comprised of 3.0% and 1.3% mature storage protein, respectively. Maximum storage protein synthesis was found to occur during early globular- and early heart-stages. During this period of development, significant levels of protein precursors were found also to accumulate. The pattern of storage protein synthesis, processing and accumulation paralleled the pattern that has been reported for the zygotic system, although somatic embryos accumulate storage protein at much earlier stages and to a lesser degree. The possibility of using complex biochemical pathways to monitor embryogenic systems in vitro is discussed.
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References
Crouch ML, Sussex IM (1981) Planta 153:64–74.
Crouch ML (1982) Planta 156:520–524.
Dure III L, Chlan C (1981) Plant Physiol 68:180–186.
Dure III L, Galau GA (1981) Plant Physiol 68:187–194.
Dure III LS, Galau GA, Greenway S (1981) Isr. J. Bot. 29:293–306.
Dure III L, Pyle JB, Chlan CA, Baker JC, Galau GA (1983) Plant Mol. Biol. 2:189–198.
Gamborg OL (1975) In: Gamborg OL, Wetter LR (eds) National Research Council of Canada, Saskatoon, Saskatchewan, pp 1–10.
Garvey JS, Gremer NE, Susdorf DH (1977) Methods in Immunology, WA Benjamin Inc., Reading, MA.
Laemmli UK (1970) Nature 227:680–685.
Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497.
Murray DR (1984) In: Murray DR (ed) Seed Physiology, vol 1, Academic Press, New York, pp 83–137.
Nitsch JP (1969) Phytomorphology 19:389–404.
Peterson GL (1977) Anal. Bioch. 83:346–356.
Pundir NS (1972) Bot. Gaz. 133:7–26.
Reeves R (1935) J. Agric. Res. 51:935–944.
Shoemaker RC, Couche LJ, Galbraith DW (1986) Plant Cell Reports 3:178–181.
Towbin H, Staehelin T, Gordan J (1979) Proc. Natl. Acad. Sci. USA 76:4350–4354.
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Communicated by J. M. Widholm
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Shoemaker, R.C., Christofferson, S.E. & Galbraith, D.W. Storage protein accumulation patterns in somatic embryos of cotton (Gossypium hirsutum L.). Plant Cell Reports 6, 12–15 (1987). https://doi.org/10.1007/BF00269728
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DOI: https://doi.org/10.1007/BF00269728