Abstract
A three year old, alkaloid producing cell line of Catharanthus roseus, maintained at 25°C, was grown on 2% sucrose at various temperatures from 10° to 45°C. Growth rates were maximal at 35°C but declined rapidly above 35°C and below 25°C. Maximum serpentine yields reached a peak at between 20°C and 25°C and fell sharply above and below these temperatures, while ajmalicine showed a sharp peak of accumulation at 20°C. The variable serpentine/ajmalicine ratio at different growth temperatures suggests that lower temperatures may favour ajmalicine accumulation. Both the growth rate and the rate of alkaloid accumulation at 25°C were therefore sensitive to small changes in average culture temperature.
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Morris, P. Regulation of product synthesis in cell cultures of Catharanthus roseus. Effect of culture temperature. Plant Cell Reports 5, 427–429 (1986). https://doi.org/10.1007/BF00269633
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DOI: https://doi.org/10.1007/BF00269633