Abstract
Shikonin derivatives were produced from cell suspension cultures of Lithospermum erythrorhizon for the first time. The results of studies on various culture media showed that the type of nitrogen source in the medium greatly influenced the production of these derivatives. When nitrate was the nitrogen source, stable production of shikonin derivatives by the cell suspension culture took place. We also found that ammonium as the nitrogen source conspicuously inhibited the production of shikonin derivatives.
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Blaydes DF (1966) Physiol. Plant. 18: 748–753
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158
Ikeda T, Matsumoto T, Noguchi M (1977) Agric. Biol. Chem. 41: 1197–1201
Linsmaier EF, Skoog F (1965) Physiol. Plant. 18: 100–127
Mizukami H, Konoshima M, Tabata M (1977) Phytochemistry 16: 1183–1186
Mizukami H, Konoshima M, Tabata M (1978) ibid. 17: 95–97
Nitsch JP, Nitsch C (1956) Amer. J. Bot. 43: 839–851
Nitsch JP, Nitsch C (1969) Science 163: 85–87
Riker AJ, Gutsch AE (1948) Amer. J. Bot. 35: 227–238
Tabata M, Mizukami H, Hiraoka N (1974) Phytochemistry 13: 927–937
White PR (1954) The Cultivation of Animal and Plant Cells, The Ronald Press Co., New York.
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Fujita, Y., Hara, Y., Ogino, T. et al. Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon . Plant Cell Reports 1, 59–60 (1981). https://doi.org/10.1007/BF00269272
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DOI: https://doi.org/10.1007/BF00269272