Abstract
Protoplasts were isolated from cotyledons and foliage leaves of cotton (Gossypium hirsutum and G. barbadense). Cotyledon protoplasts were larger and responded to culture better than leaf protoplasts. Cotyledon derived protoplasts regenerated cell walls and formed microcolonies of 2–3 cells in G. hirsutum and 5–8 cells in G. barbadense. However, the microcolonies did not grow beyond this stage. Protoplast yield and viability, cell wall regeneration and cell division were influenced by several factors, e.g., genotype, age, tissue and growth condition of donor plant, enzyme mixture and concentration, preplasmolysis period, incubation period, and culture medium.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
α-naphthaleneacetic acid
- BAP:
-
6-benzylaminopurine
- GA3 :
-
gibberellic acid
- p CPA:
-
p-chlorophenoxyacetic acid
- MES:
-
2[N-morpholino]ethanesulfonic acid
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Firoozabady, E., DeBoer, D.L. Isolation, culture, and cell division in cotyledon protoplasts of cotton (Gossypium hirsutum and G. barbadense). Plant Cell Reports 5, 127–131 (1986). https://doi.org/10.1007/BF00269251
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DOI: https://doi.org/10.1007/BF00269251