Summary
Plants resistant to Helminthosporium maydis race T were obtained following selection for H. maydis pathotoxin resistance in tissue cultures of susceptible, Texas male-sterile (T) cytoplasm maize. The selected lines transmitted H. maydis resistance to their sexual progeny as an extranuclear trait. Of 167 resistant, regenerated plants, 97 were male fertile and 70 were classified male sterile for reasons that included abnormal plant, tassel, anther or pollen development. No progeny were obtained from these male-sterile, resistant plants. Male fertility and resistance to the Phyllosticta maydis pathotoxin that specifically affects T cytoplasm maize were co-transmitted with H. maydis resistance to progeny of male-fertile, resistant plants. These three traits previously were associated only with the normal (N) male-fertile cytoplasm condition in maize. Three generations of progeny testing provided no indication that the cytoplasmic association of male sterility and toxin susceptibility had been broken by this selection and regeneration procedure. Restriction endonuclease analysis of mitochondrial DNA (mtDNA) revealed that three selected, resistant lines had distinct mtDNA organization that distinguished them from each other, from T and from N cytoplasm maize. Restriction patterns of the selected resistant lines were similar to those from T cytoplasm mtDNA; these patterns had not been observed in any previous analyses of various sources of T cytoplasm. The mtDNA analyses indicated that the male-fertile, toxin-resistant lines did not originate from selection of N mitochondrial genomes coexisting previously with T genomes in the T cytoplasm line used for selection.
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Literature
Belliard, G.; Vedel, F., Pelletier, G. (1979): Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281, 401–403
Berville, A. (1978): Characterization of mitochondria from mutants resistant to Helminthosporium maydis race T. In: Plant mitochondria (eds. Ducet, G.; Lance, C.), pp. 427–434. Amsterdam-Shannon-New York: Elsevier/North-Holland Biomedical Press
Brettell, R.I.S.; Goddard, B.V.D.; Ingram, D.S. (1979): Selection of Tms-cytoplasm maize tissue cultures resistant to Drechslera maydis T-toxin. Maydica 24, 203–312
Cassini, R.; Cornu, A.; Berville, A.; Vuillaume, E.; Panouille, A. (1977): Heredite et caracteristiques des sources de resistance a Helminthosporium maydis race T, obtenues par mutagenese chez des mais a cytoplasme male sterile Texas. Ann. Amelior. Plant. 27, 753–766
Comstock, J.C.; Martinson, C.A.; Gengenbach, B.G. (1973): Host specificity of a toxin from Phyllosticta maydis for Texas cytoplasmically male-sterile maize. Phytopathology 63, 1357–1361
Cornu, A.; Cassini, R.; Berville, A.; Vuillaume, E. (1977): Recherche par mutagenese d'une resistance a Helminthosporium maydis, race T, chez les mais a cytoplasme male-sterile Texas. In: Induced Mutations Against Plant Diseases, pp. 479–488. Vienna: Intern. Atomic Energy Agency
Forde, B.G.; Leaver, C.J. (1980): Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc. Nat. Acad. Sci. (Wash.) 77, 418–422
Forde, B.G.; Oliver, R.J.C.; Leaver, C.J. (1978): Variation in mitochondrial translation products associated with male-sterile cytoplasms in make. Proc. Nat. Acad. Sci. (Wash.) 75, 3841–3845
Gengenbach, B.G.; Green, C.E. (1975): Selection of T-cytoplasm maize callus cultures resistant to Helminthosporium maydis race T pathotoxin. Crop Sci. 15, 645–649
Gengenbach, B.G.; Green, C.E.; Donovan, C.D. (1977): Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc. Nat. Acad. Sci. (Wash.) 74, 5113–5117
Green, C.E. (1977): Prospects for crop improvement in the field of cell culture. Hort. Sci. 12, 131–134
Green, C.E.; Phillips, R.L. (1975): Plant regeneration from tissue cultures of maize. Crop Sci. 15, 417–421
Hooker, A.L.; Smith, D.R.; Lim, S.M.; Beckett, J.B. (1970): Reaction of corn seedlings with male-sterile cytoplasm to Helminthosporium maydis. Plant Dis. Reptr. 54, 708–712
Kemble, R.J.; Bedbrook, J.R. (1980): Low molecular weight circular and linear DNA molecules in mitochondria from normal and male-sterile cytoplasms of Zea mays. Nature 284, 565–566
Laughnan, J.R.; Gabay, S.J. (1973): Mutations leading to nuclear restoration of fertility in S male-sterile cytoplasm in maize. Theor. Appl. Genet. 43, 109–116
Levings, C.S., III; Pring, D.R. (1976): Restriction endonuclease analysis of mitochondrial DNA from normal and Texas cytoplasmic male-sterile maize. Science 193, 158–160
Levings, C.S., III; Pring, D.R.; Conde, M.F.; Laughnan, J.R.; Gabay-Laughnan, S.J. (1979a): The basis for cytoplasmic instability in S cytoplasm. Maize Genet. Newslett. 53, 83–84
Levings, C.S., III; Shah, D.M.; Hu, W.W.L.; Pring, D.R.; Timothy, D.H. (1979b): Molecular heterogeneity among mitochondrial DNAs from different maize cytoplasms. In: Extrachromosomal DNA (ICN-UCLA Symposia), pp. 63–74. New York: Acad. Press
Lim, S.M.; Hooker, A.L. (1972): Disease determinant of Helminthosporium maydis race T. Phytopathology 62, 968–971
Pring, D.R.; Levings, C.S., III (1978): Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89, 121–136
Pring, D.R.; Levings, C.S., III; Hu, W.W.L.; Timothy, D.H. (1977): Unique DNA associated with mitochondria in the ‘S’-type cytoplasm of male-sterile maize. Proc. Nat. Acad. Sci. (Wash.) 74, 2904–2908
Quetier, F.; Vedel, F. (1977): Heterogeneous populations of mitochondrial DNA molecules in higher plants. Nature 268, 365–368
Scheifele, G.L.; Whitehead, W.; Rowe, C. (1970): Increased susceptibility to southern leaf spot (Helminthosporium maydis) in inbred lines and hybrids of maize with Texas male-sterile cytoplasm. Plant Dis. Reptr. 54, 501–503
Singh, A.; Laughnan, J.R. (1972): Instability of S male-sterile cytoplasm in maize. Genetics 71, 607–620
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Communicated by O.E. Nelson
Scientific Journal Series Article no. 11,185 of the Minnesota Agricultural Experiment Station and no. 2295 of the Florida Agricultural Experiment Station. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee of warrantly of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable
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Gengenbach, B.G., Connelly, J.A., Pring, D.R. et al. Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theoret. Appl. Genetics 59, 161–167 (1981). https://doi.org/10.1007/BF00264970
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DOI: https://doi.org/10.1007/BF00264970