Summary
High glucose concentrations result in high levels of 2,3-butanediol, improved yield and productivity, and a decrease in cell growth in batch cultures of Klebsiella oxytoca. A maximum of 84.2 g butanediol/l and a yield of 0.5 was obtained with an initial glucose concentration of 262.6g/l. Adding the substrate in two steps in a modified fed-batch operation resulted in 85.5 g butanediol/l, 6.4 g acetoin/l and 3.4 g ethanol/l with a net yield of 0.5. Increasing the cell density to 60g/l resulted in productivities as high as 3.22 g/l.h.
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Qureshi, N., Cheryan, M. Production of 2,3-butanediol by Klebsiella oxytoca . Appl Microbiol Biotechnol 30, 440–443 (1989). https://doi.org/10.1007/BF00263847
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DOI: https://doi.org/10.1007/BF00263847