Abstract
Amino acid consumption was studied with batch and continuous chemostat cultures of Chloroflexus aurantiacus grown phototrophically in complex medium with casamino acids (Pierson and Castenholz 1974). Amino acids like Arg, Asx, Thr, Ala, Tyr, which were utilized during the early exponential phase by cells grown in batch cultures were consumed in chemostat cultures essentially at any of the dilution rates employed (0.018–0.104 h-1). Those amino acids which were taken up during subsequent phases of growth were consumed in chemostat cultures preferentially at low dilution rates. For example, the consumption of Glx was enhanced during the late exponential phase and at low dilution rates. At high dilution rates Glx was not consumed at all. Since Glx utilization largely paralleled bacteriochlorophyll formation, it is discussed that formation of the photopigment depends on the intracellular availability of Glu as the exclusive precursor for tetrapyrrole synthesis.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Avissar YJ, Ormerod JG, Beale SI (1989) Distribution of 5-aminolevulinic acid biosynthetic pathways among phototrophic bacterial groups. Arch Microbiol 151:513–519
Castenholz RW (1969) Thermophilic blue-green algae and the thermal environment. Bact Rev 33:476–504
Clayton RK (1966) Spectroscopic analyses of bacteriochlorophyll in vitro and in vivo. Photochem Photobiol 5:669–677
Feick RG, Fitzpatrick M, Fuller RC (1982) Isolation and characterization of cytoplasmic membrane and chlorosomes from the green bacterium Chloroflexus aurantiacus. J Bacteriol 150: 905–915
Foster JM, Redlinger TE, Blankenship RE, Fuller RC (1986) Oxygen regulation of development of the photosynthetic membrane system in Chloroflexus aurantiacus. J Bacteriol 167:655–659
Fuller RC, Redlinger TE (1985) Light and oxygen regulation of the development of the photosynthetic apparatus in Chloroflexus aurantiacus. In: Steinbeck KE, Bonitz S, Arntzen CL, Bogorad L (eds) Molecular biology of the photosynthetic apparatus. Cold Spring Harbor Laboratory, Cold Spring Harbor NY, pp 155–162
Golecki JR, Oelze J (1987) Quantitative relationship between bacteriochlorophyll content, cytoplasmic membrane structure and chlorosome size in Chloroflexus aurantiacus. Arch Microbiol 148:236–241
Heda GD, Madigan MT (1986) Utilization of amino acids and lack of diazotrophy in the thermophilic anoxygenic phototroph Chloroflexus aurantiacus. J Gen Microbiol 132:2469–2473
Jürgens UJ, Meißner J, Fischer U, König WA, Weckesser J (1987) Ornithine as a constituent of the peptidoglycan of Chloroflexus aurantiacus, diaminopimelic acid in that of Chlorobium vibrioforme f. thiosulfatophilum. Arch Microbiol 148:72–76
Kern M, Klemme J-H (1989) Inhibition of bacteriochlorophyll biosynthesis by gabaculin (3-amino-2,3-dihydrobenzoic acid) and presence of an enzyme of the C5-pathway of 5-aminolevulinate synthesis in Chloroflexus aurantiacus. Z Naturfosch [C] 44:77–80
Lowry OH, Rosebrough NJ, Farr AL, Randall RF (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:254–275
Oelze J, Fuller RC (1987) Growth rate and control of the development of the photosynthetic apparatus in Chloroflexus aurantiacus. Arch Microbiol 148:132–136
Pierson BK, Castenholz RW (1974) Studies of pigments and growth in Chloroflexus aurantiacus a phototrophic filamentous bacterium. Arch Microbiol 100:283–305
Pipes WO, Koutsoyannis SP (1962) Light-limited growth of Chlorella in continuous culture. Appl Microbiol 10:1–5
Schmidt K, Maarzahl M, Mayer F (1980) Development and pigmentation of chlorosomes in Chloroflexus aurantiacus OK-70-fl. Arch Microbiol 127:87–97
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oelze, J., Jürgens, U.J. & Ventura, S. Amino acid consumption by Chloroflexus aurantiacus in batch and continuous cultures. Arch. Microbiol. 156, 266–269 (1991). https://doi.org/10.1007/BF00262996
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00262996