Summary
The interrelationships of Beta chloroplast genomes have been investigated on the basis of the analysis of Fraction I protein and chloroplast (ct) DNA. Three groups of the chloroplast genomes could be demonstrated by the difference in isoelectric points of the large subunit of Fraction I protein. Restriction enzyme analysis revealed inter- and intra-specific variations among the ctDNAs, which enabled us to detect seven distinct ctDNA types. In Vulgares and Corollinae species, the observed differences were physically mapped taking advantage of the restriction fragment map available for sugar beet (B. vulgaris) ctDNA. The DNA variations were found to result either from gains or losses of restriction sites or from small deletions/ insertions, and most of them were located in the large single-copy region of the genome. Moreover, the ctDNAs from Patellares species are more diverged from those of other Beta taxa. Our results also indicate that there is a close correlation between the chloroplast genome diversity and the accepted taxonomic classification of the species included in this survey.
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Bosemark NO (1969) Interspecific hybridization in Beta L.; prospects and value in sugar beet breeding. IIRB 4:112–122
Bowman CM, Bonnard G, Dyer TA (1983) Chloroplast DNA variation between species of Triticum and Aegilops. Location of the variation on the chloroplast genome and its relevance to the inheritance and classification of the cytoplasm. Theor Appl Genet 65:247–262
Brears T, Schardl CL, Lonsdale DM (1986) Chloroplast genome organisation in sugar beet and maize. Plant Mol Biol 6:171–177
Chen K, Kung SD, Gray JC, Wildman SG (1976) Subunit polypeptide composition of Fraction I protein from various plant species. Plant Sci Lett 7:429–434
Coons GH (1954) The wild species of Beta. Proc Am Soc Sugar Beet Technol 8:142–147
Coons GH (1975) Interspecific hybrids between Beta vulgaris L. and the wild species of Beta. J Am Soc Sugar Beet Technol 18:281–306
Gordon KHJ, Crouse EJ, Bohnert HJ, Herrmann RG (1982) Physical mapping of differences in chloroplast DNA of the five wild-type plastomes in Oenothera subsection Euoenothera. Theor Appl Genet 61:373–384
Herrmann RG, Whitfeld PR, Bottomley W (1980) Construction of a SalI/PstI restriction map of spinach chloroplast DNA using low-gelling-temperature agarose electrophoresis. Gene 8:179–191
Hirai A, Tsunewaki K (1981) Genetic diversity of the cytoplasm in Triticum and Aegilops. 8. Fraction I protein of 39 cytoplasms. Genetics 99:487–493
Kishima Y, Mikami T, Harada T, Shinozaki K, Sugiura M, Kinoshita T (1986) Restriction fragment map of sugar beet (Beta vulgaris L.) chloroplast DNA. Plant Mol Biol 7:201–205
Kung SD, Sakano K, Wildman SG (1974) Multiple peptide composition of the large and small subunits of Nicotiana tabacum fraction I protein ascertained by fingerprinting electrofocusing. Biochim Biophys Acta 365:138–147
Mikami T, Sugiura M, Kinoshita T (1984a) Molecular hetero geneity in mitochondrial and chloroplast DNAs from normal and male sterile cytoplasms in sugar beets. Curr Genet 8:319–322
Mikami T, Kishima Y, Sugiura M, Kinoshita T (1984b) Chloroplast DNA diversity in the cytoplasms of sugar beet and its related species. Plant Sci Lett 36:231–235
Mikami T, Shinozaki K, Sugiura M, Kinoshita T (1984c) Characterization of chloroplast DNA from sugar beet with normal and male sterile cytoplasms. Jpn J Genet 59:497–504
Palmer JD, Thompson WF (1982) Chloroplast DNA rearrangements are more frequent when a large inverted repeated sequence is lost. Cell 29:537–550
Salts Y, Herrmann RG, Peleg N, Lavi U, Izhar S, Frankel R, Beckmann JS (1984) Physical mapping of plastid DNA variation among eleven Nicotiana species. Theor Appl Genet 69:1–14
Seyer P, Kowallik KV, Herrmann RG (1981) A physical map of Nicotiana tabacum plastid DNA including the location of structural genes for ribosomal RNAs and the large subunit of ribulose bisphosphate carboxylase/oxygenase. Curr Genet 3:189–204
Sugiura M, Kusuda J (1979) Molecular cloning of tobacco chloroplast ribosomal RNA genes. Mol Gen Genet 172:137–141
Tassopulu D, Kung SD (1984) Nicotiana chloroplast genome. 6. Deletion and hot spot — a proposed origin of the inverted repeats. Theor Appl Genet 67:185–193
Tsunewaki K, Ogihara Y (1983) The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops species. 2. On the origin of polyploid wheat cytoplasms as suggested by chloroplast DNA restriction fragment pat terns. Genetics 104:155–171
Zossimovitch VP (1940) Wild species and origin of cultivated beets. In: Panasjuk M (ed) Biology, genetics, and selection of sugar beets. All Union Sci Inst Sugar Ind Gov Publ USSR Kiev, pp 17–44
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Kishima, Y., Mikami, T., Hirai, A. et al. Beta chloroplast genomes: analysis of Fraction I protein and chloroplast DNA variation. Theoret. Appl. Genetics 73, 330–336 (1987). https://doi.org/10.1007/BF00262497
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DOI: https://doi.org/10.1007/BF00262497