Summary
The production of cellulases and of xylanase by Streptomyces lividans 1326 was studied under different growth conditions. The strain grew between 18°C and 46°C and is therefore thermotolerant. Submerged cultures of the microorganism, when grown on a defined salt medium containing xylan as main carbon source, exhibited an overall cellulolytic activity as determined by the filter paper test. S. lividans produced optimal levels of extracellular β-1,4-glucan-glucanohydrolase (1 IU/ml) and large amounts of β-1,4-xylanxylanohydrolase (50 IU/ml) at 40°C. A better production of both enzymes was observed when xylan instead of cellulose was used as substrate.
The stability of the enzyme was found to be significantly greater than those of the cellulases and xylanases produced by other streptomycetes. The optimal incubation temperatures for the enzyme assays were 55°C and 60°C for CM-cellulase and xylanase respectively and optimal pH values were found in the range of pH 6–7.
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Beldman G, Searle-van Leeuwen MF, Rombouts FM, Voragen FGJ (1985) The cellulases of Trichoderma viride. Eur J Biochem 146:301–308
Bergmeyer HU, Bernt E (1974) Determination of d-glucose with glucose oxidase and peroxidase. In: Bergmeyer HU (ed) Methods in enzyme analysis (Vol. 3) Verlag Chemie Weinheim, 2nd edition. p 1205
Catton C (1983) The case for compost. New Scientist 100:38–40
Crawford DL (1981) Microbial conversion of lignin to useful chemicals using a lignin degrading Streptomyces. Biotechnol Bioeng Symp 11:275–291
Daigneault-Sylvestre N, Kluepfel D (1979) Method for rapid screening of cellulolytic streptomycetes and their mutants. Can J Microbiol 25:858–860
Gong CS, Tsao GT (1979) Cellulases and biosynthesis regulation. In: Perlman D (ed) Ann Report of Fermentation on processes (Vol 3) Academic Press Inc NY. p 111
Hägerdahl B, Ferchack JD, Pye EK (1978) Cellulolytic enzyme system of Thermoactinomyces sp grown on microcrystalline cellulose. Appl Environ Microbiol 36:606–612
Hägerdahl B, Ferchack JD, Pye EK (1980) Saccharification of cellulose by the cellulolytic enzyme system of Thermomonospora sp. Biotechnol Bioeng 22:1515–1526
Ishaque M, Kluepfel D (1980) Cellulase complex of a mesophilic Streptomyces strain. Can J Microbiol 26:183–189
Kluepfel D, Ishaque M (1982) Xylan-induced cellulolytic enzymes in Streptomyces flavogriseus. Dev Ind Microbiol 23:389–395
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275
MacKenzie CR, Bilous D, Johnson KG (1984) Streptomyces flavogriseus cellulase: Evaluation under various hydrolysis conditions. Biotechnol Bioeng 26:590–594
Mandels M, Andreotti R, Roche C (1976) Measurements of saccharifying cellulase. Biotechnol Bioeng Symp 6:21–33
Miller GL, Blum R, Glennon WE, Burton AL (1960) Measurement of carboxymethyl cellulase activity. Anal Biochem 2:127–132
Moldoveanu N, Kluepfel D (1983) Comparison of β-glucosidase activities in different Streptomyces strains. Appl Environ Microbiol 46:17–21
Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340
Stutzenberger FJ (1972) Cellulolytic activity of Thermomonospora curvata: Nutritional requirements for cellulase production. Appl Microbiol 24:77–82
Van Zyl WH (1985) A study of the cellulases produced by three mesophilic actinomycetes grown on bagasse as substrate. Biotechnol Bioeng 27:1367–1373
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Kluepfel, D., Shareck, F., Mondou, F. et al. Characterization of cellulase and xylanase activities of Streptomyces lividans . Appl Microbiol Biotechnol 24, 230–234 (1986). https://doi.org/10.1007/BF00261542
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DOI: https://doi.org/10.1007/BF00261542