Summary
Protein synthesis-stimulating activity was assayed in the cytosolic fraction of white muscle from teleost fish (rainbow trout, carp) and of rat liver. In vitro protein synthesis-stimulating activity in the cytosolic fraction is reduced by food deprivation. The addition of elongation factors EF1, EF2, or EF1+EF2 compensates for the starvation-induced loss of protein synthesis-stimulating activity in trout muscle cytosol. The action of EF2 is stronger than that of EF1 in this respect. However, EF1 enhances in vitro protein synthesis-stimulating activity in rat liver cytosol more than EF2. The EF2 concentration in the cytosolic fraction of white muscle from starved trout is significantly lower than in fed specimens.
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Abbreviations
- EF:
-
elongation factor(s)
- SGR:
-
specific growth rate
- TCA:
-
trichloroacetic acid
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Jürss, K., Junghahn, I. & Bastrop, R. The role of elongation factors in protein synthesis rate variation in white teleost muscle. J Comp Physiol B 162, 345–350 (1992). https://doi.org/10.1007/BF00260762
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DOI: https://doi.org/10.1007/BF00260762