Summary
Phospholipase C in the antennae of Periplaneta americana was stimulated by pheromones in a GTP-dependent manner. The enzyme activity was potentiated by hydrolysis-resistant analogs of GTP and decreased by GDP analogs. Guanine nucleotide binding regulatory proteins (G-proteins) in antennal preparations were identified by bacterial toxin-catalyzed ADP-ribosylation and immunoreactivity with antibodies of designed specificity. The stimulatory effect of pheromones on inositol phosphate formation was completely blocked by pertussis toxin suggesting that the pheromone action was mediated via specific G-proteins.
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Abbreviations
- G-protein :
-
guanine nucleotide-binding regulatory protein
- IP 3 :
-
inositol 1,4,5-trisphosphate
- GTPS :
-
guanine-5′-[thio]-triphosphate
- GDPβS :
-
guanine-5′-[β-thio]-diphosphate
- NAD :
-
nicotinic acid adenine dinucleotide
- SDS-PAGE :
-
sodium dodecylsulfate polyacrylamide gel electrophoresis
- DTT :
-
dithiothreitol
- MOPS :
-
3-(N-morpholino)propanesulfonic acid
- PIPES :
-
piperazine-N,N′-bis[2-ethanesulfonic acid]
- DMSO :
-
dimethyl-sulfoxide
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Boekhoff, I., Raming, K. & Breer, H. Pheromone-induced stimulation of inositol-trisphosphate formation in insect antennae is mediated by G-proteins. J Comp Physiol B 160, 99–103 (1990). https://doi.org/10.1007/BF00258768
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DOI: https://doi.org/10.1007/BF00258768