Summary
The effect of D-mannose and D-glucose on bacteriuria due to Escherichia coli with mannose-sensitive adhesins was investigated in adult male Sprague-Dawley rats undergoing diuresis. Inocula of 105, 107, or 108 bacteria in 0.1 ml of normal saline or 2.5% or 10% D-mannose or D-glucose were injected intravesically and urine was cultured 1, 3, 5, 7 and 9 days later. The levels of bacteriuria on days 1 and 5 were significantly lower in rats inoculated with 105 E. coli and 10% D-mannose than in controls (p<0.05 and 0.01 respectively) and the percentages of rats with < 100 bacteria/ml were higher on days 1 and 3 (p=0.05 and 0.02 respectively). Bacteriuria was significantly lower in rats inoculated with 107 bacteria and 10% D-mannose than in controls on days 5 and 7 (p<0.01 for each day) and the percentage of rats with <100 bacteria/ml was higher on day 7 (p=0.01). D-glucose reduced bacteriuria significantly only with a concentration of 10% after instillation of 105 E. coli (p<0.05, day 1). The results indicate that D-mannose and D-glucose can significantly reduce bacteriuria within 1 day and that their efficacy is dependent upon the concentration of both saccharide and bacteria.
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Andriole VT, Epstein FH (1965) Prevention of pyelonephritis by water diuresis: Evidence for the role of medullary hypertonicity in promoting renal infection. J Clin Invest 44:73
Aronson M, Medalia O, Schori L, Mirelman D, Sharon N, Ofek I (1979) Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl alpha-D-mannopyranoside. J Infect Dis 139:329
Avots-Avotins AE, Fader RC, Davis CP (1981) Environmental alteration and two distinct mechanisms of E. coli adherence to bladder epithelial cells. Invest Urol 18:364
Cox CE, Hinman F Jr (1961) Experiments with induced bacteriuria, vesical emptying and bacterial growth on the mechanism of bladder defense to infection. J Urol 86:739
Dunnett CW (1980) Pairwise multiple comparisons in the homogeneous variance unequal sample size case. J Am Stat Assoc 75:789
Fowler JE, Stamey TA (1977) Studies of introital colonization in women with recurrent urinary infections. VIII. The role of bacterial adherence. J Urol 117:472
Freedman LR (1967) Experimental pyelonephritis. XIII. On the ability of water diuresis to induce susceptibility to E. coli bacteriuria in the normal rat. Yale J Biol Med 39:255
Michaels EK, Jarow JP, Schaeffer AJ (1981) Ventral bladder hernia facilitates study of urinary tract infections in rats. Urol Res 9:249
Mulholland SG (1979) Lower urinary tract antibacterial defense mechanisms. Invest Urol 17:93
Ofek I, Beachey EH (1978) Mannose binding and epithelial cell adherence of Escherichia coli. Infect Immun 22:247
Ofek I, Mirelman D, Sharon N (1977) Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature 265:623
O'Grady F, Cattell WR (1966) Kinetics of urinary tract infection. II. The bladder. Br J Urol 38:156
Orikasa SS, Hinman F Jr (1977) Reaction of the vesical wall to bacterial penetration. Resistance to attachment, desquamation and lymphocytic activity. Invest Urol 15:185
Parsons CL, Greenspan C, Mulholland SG (1975) The primary antibacterial defense mechanism of the bladder. Invest Urol 13:72
Saier MH Jr, Schmidt MR, Leibowitz M (1978) Cyclic AMP-dependent synthesis of fimbriae in Salmonella thyphimurium: Effects of cya and pts mutations. J Bacteriol 134:356
Schaeffer AJ, Amundsen SK, Jones JM (1980) Effect of carbohydrates on adherence of Escherichia coli to human urinary tract epithelial cells. Infect Immun 30:531
Schaeffer AJ, Jones JM, Dunn JK (1981) Association of in vitro Escherichia coli adherence to vaginal and buccal epithelial cells with susceptibility of women to recurrent urinary-tract infections. New Engl J Med 304:1068
Snedecor GW, Cochran WG (1967) Statistical Methods, 6th Edition, Chapter 10
Svanborg-Eden C, Eriksson B, Hanson LA, Jodal U, Kaijser B, Lidin Janson G, Lindberg U, Olling S (1978) Adhesion to normal uroepithelial cells of Escherichia coli from children with various forms of urinary tract infection. J Pediatr 93:398
Svanborg-Eden C, Hanson LA, Jodal U, Lindberg U, Sohl Akerlund A (1976) Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various froms of urinary tract Infection. Lancet II:490
Svanborg-Eden C, Jodal U (1979) Attachment of Escherichia coli to urinary sediment epithelial cells from urinary tract infection-prone and healthy children. Infect Immun 26:837
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Michaels, E.K., Chmiel, J.S., Plotkin, B.J. et al. Effect of D-mannose and D-glucose on Escherichia coli bacteriuria in rats. Urol. Res. 11, 97–102 (1983). https://doi.org/10.1007/BF00256954
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DOI: https://doi.org/10.1007/BF00256954