Summary
Alkaline phosphatase (APase) is the major protein released into the extracellular medium by strain 706, a periplasmic-excretory (lky) mutant of Escherichia coli K12. We developed a rapid three step procedure for APase purification from culture supernatants of lky mutants. Two ultrafiltration stages and an heat treatment were sufficient to obtain a 99% pure enzyme preparation. Batch culture conditions of strain 706 in a 15 l fermentor leading to an extracellular APase yield of 1250 U/ml were determined.
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Abbreviations
- APase:
-
E. coli alkaline phosphatase
References
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Atlan, D., Portalier, R. Purification of extracellular alkaline phosphatase released by Escherichia coli excretory mutants. Appl Microbiol Biotechnol 26, 318–322 (1987). https://doi.org/10.1007/BF00256661
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DOI: https://doi.org/10.1007/BF00256661