Summary
The addition of d-valine and related d-amino acids to the production medium blocks kynurenine formamidase II and actinomycin D synthesis by Streptomyces parvulus. This effect may be due to a direct inhibition of the actinomycin synthetase-catalyzed racemization of l-valine to its peptide bound d-form during antibiotic formation. In addition, the d-amino acid(s) may influence enzyme and actinomycin synthesis through carbon and/or nitrogen catabolite regulation. The relatively slow uptake and metabolism of the d-amino acid would provide a continuous supply of catabolite(s) that repress transcription of actinomycin biosynthetic genes over a long period of time.
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Arnstein HRV, Margreiter H (1958) The biosynthesis of penicillin. 7. Further experiments on the utilization of l-and d-valine and the effect of cystine and valine analogues on penicillin synthesis. Biochem J 68:339–348
Brown D, Hitchcock MJM, Katz E (1980) Evidence for a constitutive and inducible form of kynurenine formamidase in an actinomycin-producing strain of Streptomyces parvulus. Arch Biochem Biophys 202:18–22
Brown D, Hitchcock MJM, Katz E (1986) Purification and characterization of kynurenine formamidase activities from Streptomyces parvulus. Can J Microbiol 32:465–472
Demain AL (1956) Inhibition of penicillin formation by amino acid analogs. Arch Biochem Biophys 64:74–79
DiGirolamo M, Ciferri O, DiGirolamo AB, Albertini A (1964) Effect of d-leucine on the biosynthesis of polymixin D. J Biol Chem 239:502–507
Kamal F, Katz E (1976) Studies of etamycin biosynthesis employing short-term experiments with radiolabeled precursors. J Antib 29:944–949
Katz E (1980) Kynurenine formamidase isozymes in Streptomyces parvulus. In: Hayaishi O, Ishimura Y, Kido R (eds) Biochemical and Medical Aspects of Tryptophan Metabolism, Elsevier/North-Holland Biomedical Press, Amsterdam, pp 159–177
Katz E (1960) Influence of valine, isoleucine and related compounds on actinomycin synthesis. J Biol Chem 235: 1090–1094
Katz E, Goss WA (1959) Controlled biosynthesis of acinomycin with sarcosine. Biochem J 73:458–465
Katz E, Waldron CR, Meloni ML (1961) Role of valine and isoleucine as regulators of actinomycin peptide formation by Streptomyces chrysomallus. J Bacteriol 82:600–608
Katz E, Weissbach H (1963) Incorportation of 14C-labeled amino acids into actinomycin and protein by Streptomyces antibioticus. J Biol Chem 238:666–675
Lowry OH, Rosebrough JN, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Martin JF, Demain AL (1980) Control of antibiotic biosynthesis. Microbiol Rev 44:230–251
Naftalin L (1948) Quantitative chromatographic estimation of α-amino acids. Nature 161:763
Williams KW, Katz E (1977) Development of a chemically defined medium for the synthesis of actinomycin D by Streptomyces parvulus. Antimicrob Agents Chemother 11:281–290
Yajima T, Grigg MA, Katz E (1972) Biosynthesis of antibiotic peptides with isoleucine stereoisomers. Arch Biochem Biophys 151:565–575
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Katz, E., Brown, D. A possible role of d-valine and related d-amino acids in repression of enzyme and actinomycin synthesis. Appl Microbiol Biotechnol 30, 67–70 (1989). https://doi.org/10.1007/BF00255998
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DOI: https://doi.org/10.1007/BF00255998