Summary
Corynebacterium glutamicum was used in fed-batch fermentation for glutamate production. Both intracellular and extracellular concentrations were determined which allowed us to study the repartition of the amino acid according to the culture conditions and in the presence or absence of surfactants. A decrease in cell volume was observed after addition of surfactants during the exponential phase of growth; glutamate accumulates in the cell, whereas in standard industrial conditions the glutamate concentration in the medium during the production phase can be 30-fold higher than that found inside the cell. The level of excretion is compatible with industrial production.
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References
Beutler HO, Michal G (1974) l-glutamate determination with glutamate dehydrogenase, diaphorase and tetrazolium salts. In: Bergmeyer HU (ed) Methods of enzymatic analysis 2nd edition Vol. 4. Verlag Chemie, Weinheim/Bergstr., pp 1708–1713
Clement Y, Escoffier B, Trombe MC, Laneelle G (1984) Is glutamate excreted by its uptake system in Corynebacterium glutamicum? A working hypothesis. J Gen Microbiol 130:2589–2594
Demain AL, Birnbaum J (1968) Alteration of permeability for the release of metabolites from the microbial cell. Current Topics Microbiol 46:1–25
Fukui S, Ishida M (1973) Biomembrane permeability and the accumulation of amino acids. In: Yamada K (ed) The microbial production of amino acids. John Wiley, pp 123–137
Huchenq A, Marquet M, Welby M, Montrozier H, Goma G, Laneelle G (1984) Glutamate excretion triggering mechanism: a reinvestigation of the surfactant induced modification of cell lipids. Ann Microbiol (Inst. Pasteur) 135B:53–67
Kinoshita S, Tanaka K (1973) Glutamic acid. In: Yamada K (ed) The microbial production of amino acids. John Wiley, pp 263–324
Krulwich TA, Gruffanti AA, Maudel KG (1981) The role of the membrane in the bioenergetics of bacterial cells. In: Organization of prokaryotic cell membranes. Vol 2. Ghosh RH (ed) C.R.C. Press, Inc., pp 53–71
Shiio I, Narui K, Yabaha N, Takahashi M (1962) Free intracellular amino acid of a glutamate-forming Bacterium, Brevibacterium flavum No 2247. J Biochem 51:109–111
Takinami K, Yoshii H, Tsuri H, Okada H (1965) Biochemical effects of fatty acid and its derivatives on l-glutamic acid fermentation. III Biotin-tween 60 relationship in the accumulation of l-glutamic acid and the growth of Brevibacterium lactofermentum. Agric Biol Chem 29:351–359
Uribelarrea JL, Pacaud S, Goma G (1985) New method for measuring the cell water content by thermogravimetry. Biotechnol Lett 7:75–80
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Marquet, M., Uribelarrea, J.L., Huchenq, A. et al. Glutamate excretion by Corynebacterium glutamicum: a study of glutamate accumulation during a fermentation course. Appl Microbiol Biotechnol 25, 220–223 (1986). https://doi.org/10.1007/BF00253652
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DOI: https://doi.org/10.1007/BF00253652