Abstract
Washed everted vesicles of the methanogenic bacterium strain Gö1 catalyzed an H2-dependent reduction of the heterodisulfide of HS-CoM (2-mercaptoethanesulfonate) and HS-HTP (7-mercaptoheptanoylthreonine phosphate) (CoM-S-S-HTP). This process was independent of coenzyme F420 and was coupled to proton translocation across the cytoplasmic membrane into the lumen of the everted vesicles. The maximal H+/CoM-S-S-HTP ratio was 2. The tranmembrane electrochemical gradient thereby generated was shown to induce ATP synthesis from ADP+Pi, exhibiting a stoichiometry of 1 ATP synthesized per 2 CoM-S-S-HTP reduced (H+/ATP=4). ATP formation was inhibited by the uncoupler 3,5-di-tert-butyl-4-hydroxy-benzylidene-malononitrile (SF 6847) and by the ATP synthase inhibitor N,N′-dicyclohexylcarbodiimide (DCCD). This energy-conserving system showed a stringent coupling. The addition of HS-CoM and HS-HTP at 1 mM each decreased the heterodisulfide reductase activity to 50% of the control. Membranes from Methanolobus tindarius showed F420H2-dependent but no H2-dependent heterodisulfide oxidoreductase activity. Neither of these activities was detectable in membranes of Methanococcus thermolithotrophicus.
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Abbreviations
- Δμ H+:
-
transmembrane electrochemical gradient of H+
- CoM-SH:
-
2-mercaptoethanesulfonate
- F420 :
-
(N-l-lactyl-γ-l-glutamyl)-l-glutamic acid phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin-5′-phosphate
- F420H2 :
-
reduced F420
- HTP-SH:
-
7-mercaptoheptanoylthreonine phosphate
- DCCD:
-
N,N′-dicyclohexylcarbodiimide
- SF 6847:
-
3,5-di-ert-butyl-4-hydroxybenzylidenemalononitrile
- Mb. :
-
Methanobacterium
- Ml. :
-
Methanolobus
- Mc. :
-
Methanococcus
- MV:
-
methylviologen
- BV:
-
benzylviologen
- MTZ:
-
metronidazole
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Deppenmeier, U., Blaut, M. & Gottschalk, G. H2: heterodisulfide oxidoreductase, a second energy-conserving system in the methanogenic strain Gö1. Arch. Microbiol. 155, 272–277 (1991). https://doi.org/10.1007/BF00252211
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DOI: https://doi.org/10.1007/BF00252211