Summary
The serum activities of two lysosomal enzymes, β-N-acetylglucosaminidase (EC 3.2.1.30, NAG) and β-glucuronidase (EC 3.2.1.31, GLU), were determined in 41 insulin-dependent diabetics, 27 age-matched non-diabetic first-degree relatives of the diabetics and 103 age-matched non-diabetic blood-donors. The diabetics were divided into three groups on the basis of ophthalmoscopy: (1) no retinal abnormalities; (2) non-proliferative retinopathy; and (3) proliferative retinopathy. The activities of both serum enzymes were higher in diabetics (NAG 21.39 ±5.99; GLU 2.19±1.01) than in their relatives (NAG 17.22±3.99; GLU 1.62±0.61). The diabetics with non-proliferative retinopathy had higher serum enzyme levels (NAG 24.05±6.26; GLU 2.60 ±1.06) than diabetics without retinopathy (NAG 17.88±3.00; GLU 1.69±0.64), whereas no statistically significant difference was found in patients with the proliferative form of retinopathy (NAG 18.67±6.28; GLU 1.99±1.04). In diabetics a positive correlation was found between serum β-N-acetylglucosaminidase activity and blood glucose (p < 0.01), but not between β-glucuronidase and blood glucose. Furthermore, the activities of both enzymes in diabetics correlated with the plasma triglyceride level (p<0.05 for both correlations). No correlation was found between the enzyme levels and signs of other diabetic late complications.
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Andreani D, DiMario U, Iavicoli M, Pozzilli P, Lumbroso B, Guy K, Irvine WJ (1978) Immune complexes and diabetic retinopathy. Diabetologia 15: 215
Belfiore F, Napoli E, Vecchio LL (1972) Serum N-acetyl-beta-glucosaminidase activity in diabetic patients. Diabetes 21: 1168–1172
Belfiore F, Napoli E, Vecchio LL, Rabuazzo AM (1974) Increased serum activity of beta-n-acetyl-glucoseaminidase in atherosclerosis. Am J Med Sci 268: 235–239
Belfiore F, Vecchio LL, Napoli E (1972) Serum beta glucuronidase activity in diabetic patients. Am J Med Sci 264: 457–465
Belfiore F, Vecchio LL, Napoli E (1973) Serum enzymes in diabetes mellitus. Clin Chem 19: 447–452
Bomback FM, Nakagawa S, Kumin S, Nitowsky HM (1976) Altered lysosomal glycohydrolase activities in juvenile diabetes mellitus. Diabetes 25: 420–427
Borthwick LJ, Whiting PH (1978) β-N-Acetyl-glucosaminidase (β-NAG) in diabetes mellitus. Diabetologia 15: 221
Desai ID (1969) Regulation of lysosomal enzymes. I. Adaptive changes in enzyme activities during starvation and refeeding. Can J Biochem 47: 785–790
Fishman WH (1970) β-Glucuronidase. In: Bergmeyer HU (ed) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim, p 885–889
Fushimi H, Tarui S (1976) β-glycosidases and diabetic microangiopathy I. Decreases of β-glycosidase activities in diabetic rat kidney. J Biochem (Tokyo) 79: 265–270
Fushimi H, Tarui S (1976) Retina, tear and serum β-N-acetylglucosaminidase activities in diabetic patients. Clin Chim Acta 71: 1–8
Hagenfeldt L, Wahlberg F (1965) Serum β-glucuronidase, glucose tolerance, and atherosclerotic disease. Lancet I: 788–789
Holland LJ, Felix RH, Alston WC (1978) Serum N-acetyl-β-D-glucosaminidase and circulating immune complex activities in systemic lupus erythematosus. Br Med J I: 1027
Huang T, Chen C, Wefler V, etal (1961) A stable reagent for the Liebermann-Burchard reaction. Application to rapid serum cholesterol determination. Anal Chem 33: 1405–1406
Juretić D, Lipovac K, Hadzija M, Slijepcevic M (1978) The activities of glycosidases in diabetic rats. Diabetologia 15: 243
Kessler G, Lederer H (1965) In: Skeggs LI (ed) Automation in analytical chemistry. Technicon Symposia. New York p 341–344
Lewy GA, Conchie J (1966) Mammalian glycosidases and their inhibition by aldonolactones. In: Neufeld EF, Ginsburg V (eds) Methods in enzymology. Academic Press, New York, p 571–584
Miller BF, Keyes FP, Curreri PW (1966) Increase of serum β-glucuronidase activity in human diabetes mellitus. JAMA 195: 127–130
Miller BF, Keyes EP, Curreri PW (1967) Increased activity of serum β-glucuronidase in patients with coronary artery disease. J Atheroscler Res 7: 591–600
Reckless JPD, Betteridge DJ, Wu P, Payne B, Galton DJ (1978) High-density and low-density lipoproteins and prevalence of vascular disease in diabetes mellitus. Br Med J I:883–886
Siest G, Bagrel A, Panek E, Galteau MM, Batt AM, Schiele F (1973) Plasma Enzymes — Physiological and environmental variations. In: Proc. 2nd int. colloquium “Automatisation and prospective biology”. Karger, Basel, p 28–38
Siest G, Schiele F, Galteau MM, Panek E, Steinmetz J, Fagnani F, Gueguen R (1975) Aspartate aminotransferase and alanine aminotransferase activities in plasma. Statistical distributions, individual variations, and reference values. Clin Chem 21: 1077–1087
Tulsiani DRP, Buschiazzo HO, Tolbert B, Touster O (1977) Changes in plasma hydrolase activities in hereditary and streptozotocin-induced diabetes. Arch Biochem Biophys 181: 216–227
Wissenschaftliche Tabellen (1960) 6. Auflage. Geigy, Basel
Woollen JW, Turner P (1965) Plasma N-acetyl-β-glucuronidase in health and disease. Clin Chim Acta 12: 671–683
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Pitkänen, E., Kyllästinen, M., Koivula, T. et al. β-N-acetylglucosaminidase and β-glucuronidase activities in insulin-dependent diabetic subjects with retinopathy. Diabetologia 18, 275–278 (1980). https://doi.org/10.1007/BF00251004
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DOI: https://doi.org/10.1007/BF00251004