Summary
Parvalbumin mRNA was localized in rat brain by in situ hybridization using a 35S labelled rat parvalbumin cDNA and a synthetic oligodeoxyribonucleotide (corresponding to base sequences 140 to 183 of rat parvalbumin cDNA). Strongest hybridization signals were detected in the Purkinje cells of the cerebellum and in neurones of the reticular nucleus of the thalamus. Signal was also detected in the cerebral cortex, hippocampus, basal ganglia and brain stem in agreement with the distribution of parvalbumin immunoreactivity.
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Arai H, Emson PC, Mountjoy CQ, Carassco LH, Heizmann CW (1987) Loss of parvalbumin-immunoreactive neurones from cortex in Alzheimer-type dementia. Brain Res 418: 164–169
Berchtold MW (1987) Cloning and expression of the gene encoding the Ca2+-binding protein parvalbumin. In: Means AR, Conn PM (eds) Methods in enzymol, Vol 139. Academic Press, San Diego, pp 317–325
Berchtold MW, Means AR (1985) The Ca2+-binding protein parvalbumin: molecular cloning and developmental regulation of mRNA abundance. Proc Natl Acad Sci USA 82: 1414–1418
Berchtold MW, Epstein P, Beaudet AL, Payne ME, Heizmann CW, Means AR (1988) Structural organisation and chromosomal assignment of the parvalbumin gene. J Biol Chem 262: 8696–8701
Celio MR (1986) Parvalbumin in most-aminobutyric acid-containing neurones of the rat cerebral cortex. Science 231: 995–997
Celio MR, Heizmann CW (1981) Calcium-binding protein parvalbumin as a neuronal marker. Nature 293: 300–302
Crapper McLachlan DR, Wong L, Bergeron C, Baimbridge KG (1987) Calmodulin and calbindin D28K in Alzheimer disease. Alzheimer Disease and Associated Disorders 3: 171–179
Endo T, Kobayashi S, Onata T (1985) Parvalbumin in rat cerebrum, cerebellum and retina during postnatal development. Neurosci Lett 60: 279–282
Epstein P, Means AR, Berchtold MW (1986) Isolation of a rat parvalbumin gene and full length cDNA. J Biol Chem 261: 5886–5891
Feldman SC, Christakos S (1983) Vitamin D-dependent calciumbinding protein in rat brain: biochemical and immunochemical characterization. Endocrinology 112: 290–302
Gibson GE, Peterson C (1987) Changes in calcium homeostasis during ageing, in modification of cell to signals during normal and pathological ageing. In: Govoni S, Battaini F (eds) NATO-ASI series, Vol 19. Springer, Berlin, pp 121–140
Heizmann CW (1984) Parvalbumin, an intracellular calciumbinding protein; distribution, properties and possible roles in mammalian cells. Experientia 40: 910–921
Heizmann CW, Berchtold MW (1987) Expression of parvalbumin and other Ca2+-binding proteins in normal and tumor cells: a topical review. Cell Calcium 8: 1–41
Heizmann CW, Celio MR (1987) Immunolocalization of parvalbumin. In: Means AR, Conn PM (eds) Methods in enzymol, Vol 139. Academic Press, New York, pp 552–570
Houser CR, Vaughn JE, Barber RP, Roberts E (1980) GABA-neurones are the major cell type of the nucleus reticularis thalami. Brain Res 200: 341–354
Jande SS, Maler L, Lawson DEM (1981) Immunohistochemical mapping of vitamin D-dependent calcium-binding protein in brain. Nature 294: 765–767
Kagi U, Berchtold MW, Heizmann CW (1987) Ca2+-binding parvalbumin in rat testis: characterization, localization and expression during development. J Biol Chem 15: 7314–7320
Kawaguchi Y, Katsumara H, Kosaka T, Heizmann CW, Hama K (1987) Fast spiking cells in rat hippocampus (CA1 region) contain the calcium-binding protein, parvalbumin. Brain Res 416: 369–374
Kosaka T, Katsumaru H, Kawaguchi Y, Hama K, Wu J-Y, Heizmann CW (1987) GABAergic neurones containing the Ca++-binding protein parvalbumin in the rat hippocampus and dentate gyrus. Brain Res 419: 119–130
Mayer ML, Westbrook GL (1987) Cellular mechanisms underlying excitotoxicity. Trends Neurosci 10: 59–61
Mugnaini E, Oertel WH (1983) An atlas of the distribution of GABAergic neurones and terminals in the rat CNS as revealed by GAD immunohistochemistry. In: Björklund A, Hökfelt T (eds) GABA and neuropeptides in the CNS. Handbook of chemical neuroanatomy, Vol 4, Part 1. Elsevier, Amsterdam, pp 436–608
Rogers JH (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurones. J Cell Biol 105: 1343–1353
Shivers BD, Schachter BS, Pfaff DW (1987) In situ hybridization for the study of gene expression in the brain. In: Conn PM (ed) Methods in enzymology, Vol 124. Academic Press, New York, pp 497–510
Stichel CC, Singer W, Heizmann CW, Norman AW (1987) Immunohistochemical localization of calcium-binding proteins, parvalbumin and calbindin-D28K, in the adult and developing visual cortex of cats: a light and electron microscopic study. J Comp Neurol 262: 563–577
Stichel CC, Singer W, Heizmann CW (1988) Light and electron microscopic immunocytochemical localization of parvalbumin in the dorsal lateral geniculate nucleus of the cat: evidence for co-existence with GABA. J Comp Neurol 268: 29–37
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Seto-Ohshima, A., Emson, P.C., Berchtold, M.W. et al. Localization of parvalbumin mRNA in rat brain by in situ hybridization histochemistry. Exp Brain Res 75, 653–658 (1989). https://doi.org/10.1007/BF00249917
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DOI: https://doi.org/10.1007/BF00249917