Abstract
Single channel performance and deactivation currents have been analyzed in the presence of cation channel blockers to reveal pharmacological properties of the slow-activating (SV) cation-selective ion channel in the vacuolar membrane (tonoplast) isolated from suspension cells of Chenopodium rubrum L. At a holding potential of −100 mV, the SV channel showed half-maximal inhibition with 20mm tetraethylammonium (TEA), 7 μm 9amino-acridine, 6 μm (+)-tubocurarine, 300nm quinacrine, and 35 μm quinine, respectively. The SV channel is also blocked by charybdotoxin (20nm at −80 mV) but not by apamine. 9-Amino-acridine, (+)-tubocurarine and quinacrine act in a voltage-dependent fashion, binding to the open channel and to different sites along the transmembrane voltage profile according to Woodhull (J. Gen. Physiol. 61:687–708, 1973). No binding site could be specified for charybdotoxin, which binds to the closed channel, and for quinine. Except for quinine, all tested blockers were effective only if added to the cytoplasmic side of the tonoplast. A structural relationship between the SV channel and Maxi-K channels in animal systems is inferred.
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We are grateful to Prof. F. Dreyer and Dr. J. Beise from the Pharmacology Department of the Justus-Liebig-Universität Giessen for continuous interest and helpful suggestions. This work was supported by a grant from the Deutsche Forschungsgemeinschaft (Be 466/21-5) and the Bundesminister für Forschung und Technologie, Bonn.
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Weiser, T., Bentrup, FW. Pharmacology of the SV channel in the vacuolar membrane of chenopodium rubrum suspension cells. J. Membarin Biol. 136, 43–54 (1993). https://doi.org/10.1007/BF00241488
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DOI: https://doi.org/10.1007/BF00241488