Abstract
The subcellular compartmentation of isopentenyl diphosphate (IPP) synthesis was examined in secretory cells isolated from glandular trichomes of peppermint (Mentha x piperita L. cv. Black Mitcham). As a consequence of their anatomy and the conditions of their isolation, the isolated secretory cells are non-specifically permeable to low-molecular-weight water-soluble metabolites. Thus, the cytoplasm is readily accessible to the exogenous buffer whereas the selective permeability of subcellular organelles is maintained. With the appropriate choice of exogenous substrates, this feature allows the assessment of cytoplasmic and organellar (e.g. plastidic) metabolism in situ. Glycolytic substrates such as [14C]glucose-6-phosphate and [14C]pyruvic acid are incorporated into both monoterpenes and sesquiterpenes with a monoterpene:sesquiterpene ratio that closely mimics that observed in vivo, indicating that the correct subcellular partitioning of these substrates is maintained in this model system. Additionally, exogenous [14C]mevalonic acid and [14C]IPP, which are both intitially metabolized in the cytoplasm, produce an abnormally high proportion of sesquiterpenes. In contrast, incubation with either [14C]citrate or [14C]acetyl-CoA results in the accumulation of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) with no detectable isoprenoids formed. Taken together, these results indicate that the cytoplasmic mevalonic acid pathway is blocked at HMG-CoA reductase and that the IPP utilized for both monoterpene and sesquiterpene biosynthesis is synthesized exclusively in the plastids.
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Abbreviations
- DMAPP:
-
dimethylallyl diphosphate
- FPP:
-
farnesyl diphosphate
- GLC:
-
gas liquid chromatography
- GPP:
-
geranyl diphosphate
- HMG-CoA:
-
3-hydroxy-3-methylglutaryl-CoA
- IPP:
-
isopentenyl diphosphate
- PEP:
-
phosphoenolpyruvic acid
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This investigation was supported in part by U.S. Department of Energy Grant DE-FG06-88ER 13869, the Mint Industry Research Council, and Project 0268 from the Agricultural Research Center, Washington State University. We thank Jonathan Gershenzon for helpful criticisms of the manuscript and Greg Wichelns for growing the plants.
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McCaskill, D., Croteau, R. Monoterpene and sesquiterpene biosynthesis in glandular trichomes of peppermint (Mentha x piperita) rely exclusively on plastid-derived isopentenyl diphosphate. Planta 197, 49–56 (1995). https://doi.org/10.1007/BF00239938
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DOI: https://doi.org/10.1007/BF00239938