Abstract
We have obtained fertile transgenic plants of Indica rice variety IR36, by using electroporation to transfer the neomycin phosphotransferase II (nptII) gene into cells of mature embryos. Resistant calli were selected in the presence of 30 μg/ml G418. Nearly thirty transgenic plants were regenerated within three months after transformation. Many of them yielded seeds following self-pollination. Data from molecular analysis and enzyme assay proved that the foreign gene was stably integrated into the genome of resistant calli, R0 and R1 plants, and also expressed. Mendelian segregation of the nptII gene was observed in R1 progeny plants.
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Abbreviations
- NOS:
-
nopaline synthase
- NPTII and nptII :
-
neomycin phosphotransferase II
- OCS:
-
octopine synthase
- Km:
-
kanamycin
References
Bradford MM (1976) Anal Biochem 72: 248–254
Christou P, Ford TL, Kofron M (1991) Bio/Technology 9: 957–962
Datta SK, Peterhans A, Datta K, Potrykus I (1990) Bio/Technology 8: 736–740
Davey MR, Kothari SL, Zhang H, Rech EL, Cocking EC, Lynch FT (1991) J Exp Bot 42: 1159–1169
Dekeyser RA, Claes B, De Rycke RMU, Habets ME, Van Montagu MC, Caplan PB (1990) The Plant Cell 2: 591–602
D'Halluin K, Bonne E, Bossut M, De Beuckeleer M, Leemans J (1992) The Plant Cell 4: 1495–1505
Feldmann KA, Marks MD (1987) Mol Gen Genet 208: 1–9
Fromm ME, Taylor LP, Walbot V (1985) Proc Natl Acad Sci USA 82: 5824–5828
Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Bio/Technology 8, 833–839
Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) The Plant Cell 2, 603–618
Hain R, Stabel P, Czernilofsky AP, Steinbiss HH, Herrera-estrella L, Schell J (1985) Mol Gen Genet 199, 166–168
Klein TM, Wolf ED, Wu R, Sanford JC (1987) Nature 327: 70–73
Klein TM, Harper EC, Svab Z, Sanford JC, Fromm ME, Maliga P (1988) Proc Natl Acad Sci USA 85: 8502–8505
Langridge WHR, Li BJ, Szalay AA (1985) Plant Cell Rep 4: 355–359
Li BJ, Xu XP, Shi HP, Ke XY (1991) Science in China (Scientia Sinica, Series B) 34: 923–931
Morikawa H, Iida A, Matsui C, Ikegami M, Yamada Y (1986) Gene 41: 121–124
Paszkowski J, Shillito RD, Saul MW, Mandak V, Hohn T, Hohn B, Potrykus I (1984) EMBO J 3: 2717–2722
Potrykus I (1990) Bio/Technology 8: 535–542
Rhodes CA, Pierce DA, Mettler U, Mascarenhas D, Detmer JJ (1988) Science 240: 204–207
Sambrook J, Fritsch EF, Maniatis (1989) Molecular cloning: A laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Shimamoto K, Terada R, Izawa T, Fujimoto H (1989) Nature 338: 274–277
Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Bio/Technology 6: 1072–1074
Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Bio/Technology 10, 66–73
Wang JF, Li BJ (1989) In: Yin XZ, Zhang Q (eds) The symposium of plant genetics molecular genetics and gene engineering, Supplement to the Acta Scientiarum Naturalium Universitatis Sunyatseni, Vol 8, pp166–169
Zhang HM, Yang H, Rech EL, Golds TJ, Davis AS, Mulligan BJ, Cocking EC, Davey MR (1988) Plant Cell Rep 7: 379–384
Zhang W, Wu R (1988) Theor Appl Genet 76: 835–840
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Xu, X., Li, B. Fertile transgenic Indica rice plants obtained by electroporation of the seed embryo cells. Plant Cell Reports 13, 237–242 (1994). https://doi.org/10.1007/BF00239900
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DOI: https://doi.org/10.1007/BF00239900