Abstract
Randomly amplified polymorphic DNA (RAPD) markers have been used to study the genetic variation among androgenetic monoploids of diploid Solanum species. Cluster analysis of pairwise genetic distances was used to construct a genetic relationship among anther donor and anther-derived potato plants. The clustering based on Rogers' distances resembled classifications based on parental origins and hybrid combinations. Six of the 32 RAPD primers used resulted in the selective amplification of DNA fragments which were polymorphic between the two S. phureja parental clones, 1.22 and A95. It should be possible to construct a genetic linkage map, without making crosses, using monoploids derived from a single heterozygous diploid clone and RAPD markers.
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References
Chen CC, Chiu WL, Yu LJ, Ren SS, Yu WJ (1983) Can J Genet Cytol 25: 18–24
Cowen NM, Johnson CD, Armstrong K, Miller M, Woosley A, Pescitelli S, Skokut M, Belmar S, Petolino JF (1992) Theor Appl Genet 84: 720–724
Doyle JJ, Doyle JL (1987) Phytochemical Bull 19: 11–15
Guiderdoni E (1991) Theor Appl Genet 81: 406–412
Klein-Lankhorst RM, Vermunt A, Weide R, Liharska T, Zabel P (1991) Theor Appl Genet 83: 108–114
Messmer MM, Melchinger AE, Lee M, Woodman WL, Lee LA, Lamkey KR (1991) Theor Appl Genet 83: 97–107
Ozias-Akins P, Lubbers EL, Hanna WW, McNay JW (1993) Theor Appl Genet (in press)
Paran I, Kasseli R, Michelmore R (1991) Genome 34: 1021–1027
Petolino JF, Jones AM (1986) Crop Sci 26: 1072–1074
Quiros CF, Hu J, This P, Chevre AM, Delseny M (1991) Theor Appl Genet 82: 627–632
Rivard SR, Cappadocia M, Vincent G, Brisson N, Landry BS (1989) Theor Appl Genet 78: 49–56
SAS Institute, Cary, North Carolina (1989)
Singsit C, Veilleux RE (1989) Euphytica 43: 105–112
Singsit C, Veilleux RE, Sterrett SB (1990) Genome 33: 50–56
Srivastava PS, Johri BM (1988) J Pathology 23: 83–99
Tulsieram LK, Glaubitz JC, Kiss G, Carlson JE (1992) Bio/Tech 10: 686–690
Uhrig H, Salamini F (1987) Plant Breed 98: 228–235
Uijtewaal BA, Huigen DJ, Hermsen JGTh (1987) Theor Appl Genet 73: 751–758
Veilleux RE, Booze-Daniels J, Pehu E (1985) Can J Genet Cytol 27: 559–564
Wenzel G, Uhrig H (1981) Theor Appl Genet 59: 333–340
Wilde J, Waugh R, Powell W (1992) Theor Appl Genet 83: 871–877
Williams JGK, Kubelik AR, Livak KJ, Tingey SV (1990) Nucleic Acid Res 18: 6531–6535
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Communicated by G.C. Phillips
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Singsit, C., Ozias-Akins, P. Genetic variation in monoploids of diploid potatoes and detection of clone-specific random amplified polymorphic DNA markers. Plant Cell Reports 12, 144–148 (1993). https://doi.org/10.1007/BF00239095
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DOI: https://doi.org/10.1007/BF00239095