Summary
By use of an anti-gastrin serum and colloidal gold- or ferritin-labelled sheep anti-rabbit γ-globulins, nerve fibres and nerve terminals containing a gastrin-like substance were characterized at the ultrastructural level in the median eminence of Xenopus laevis. These immunoreactive fibres contain neurosecretory granules displaying medium to high electron density and a mean diameter of 75 nm. Labelling intensity varies from granule to granule. This is the first demonstration at the ultrastructural level of the precise location of a gastrin-like hormone in the median eminence of a vertebrate.
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Supported by the D.G.R.S.T., Contrat no 80.7.0242
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Doerr-Schott, J., Garaud, J.C. Ultrastructural Identification of gastrin-like immunoreactive nerve fibres in the brain of Xenopus laevis by means of colloidal gold or ferritin immunocytochemical methods. Cell Tissue Res. 216, 581–589 (1981). https://doi.org/10.1007/BF00238653
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DOI: https://doi.org/10.1007/BF00238653