Abstract
There is potential to accelerate cultivar development with a doubled haploid system for breeding line production. Anther culture methodology was evaluated for U.S.A. spring barley (Hordeum vulgare L.) breeding applications. Gelrite was found to be an acceptable replacement for ficoll in the induction medium to reduce costs while maintaining embryoid and plant production levels. Beneficial effects of 28 d cold pretreatment of donor spikes for anther culture were confirmed with Pacific Northwest USA barley genotypes. A 3 d mannitol solution pretreatment of fresh anthers was shown to be less effective for green plant production compared to 28 d cold pretreatment of donor spikes. Extended donor spike cold pretreatment from 28 to 42 d did not reduce anther culture productivity. Based on this research, anther culture techniques show promise for economical and convenient application in spring barley breeding.
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Abbreviations
- DH:
-
doubled haploid
- LS:
-
Linsmaier and Skoog basal medium
- BAP:
-
benzylaminopurine
- GLM:
-
Generalized Linear Model
- SAS:
-
Statistical Analysis System
References
Flehinghaus T, Deimling S, Geiger HH (1991) Plant Cell Rep 10:397–400
Hou L, Ullrich SE (1990) Barley Newsletter 33:115 (Abstr)
Huang B, Sunderland N (1982) Ann Bot 49:77–88
Hunter CP (1985) Plant Cell Rep 4:267–268
Hunter CP (1988) PhD Thesis, Wye College, University of London
Kao KN (1981) Z Pfl Physiol 103:437–443
Kao KN, Saleem M, Abrams S, Pedras M, Horn D, Mallard C (1991) Plant Cell Rep 9:595–601
Kasha KJ, Cho UA, Ziauddin A (1992) In: Munck L (ed.) Barley Genet VI vol II. Proc Sixth Int Barley Genet Symp, July 22–27, 1991 Helsingborg, Sweden, Munksgaard Int Publ Ltd, Copenhagen pp. 793–806
Kuhlmann U, Foroughi-Wehr B, (1989) Plant Cell Rep 8:71–81
Luckett DJ, Smithard RA (1992) Aust J Agric Res 43:67–78
Lyne RL, Bennett RI, Hunter CP (1986) In: Withers LA, Alderson PG (eds) Plant Tissue Culture and Its Agricultural Application. Univ of Nottingham. Butterworth, Guildford pp. 405–411
Olsen FL (1987) Carlsberg Res Commun 52:393–404
Pickering RA, Devaux P (1992) In: Sheury PR (ed) Barley Genetics, Molecular Biology and Biotechnology. C.A.B. Intl, Wallingford, UK pp. 519–547
Powell W (1988) Plant Cell, Tissue and Organ Culture 12:291–297
SAS (1988) SAS Institute Inc., Cary, NC, USA
Sorvari S (1986) Ann Agric Fenn 25:249–254
Sunderland N, Huang B, Hills GJ (1984) J Exp Bot 85:521–530
Szarejko I, Kasha KJ (1991) Cereal Res Comm 19:219–237
Zhou H (1990) MS thesis Washington State Univ, Pullman
Zhou H, Zheng Y, Konzak CF (1991) Plant Cell Rep 10:63–66
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Communicated by G. C. Phillips
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Hou, L., Ullrich, S.E., Kleinhofs, A. et al. Improvement of anther culture methods for doubled haploid production in barley breeding. Plant Cell Reports 12, 334–338 (1993). https://doi.org/10.1007/BF00237430
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DOI: https://doi.org/10.1007/BF00237430