Summary
The effects have been analyzed of cytochalasin B and colchicine on the secretion of glycoconjugates by human bronchial expiants labeled in vitro with radioactive glucosamine. Both cytochalasin B and colchicine had no effect on baseline 14C-labeled glycoconjugate release but caused a dose-dependent (10−7–10−4 M) inhibition of 14C-glycoconjugate release and discharge of labeled macromolecules from mucous and serous cells induced by 5 · 10−5 M methacholine.
Quantitative autoradiographic analyses showed that neither cytochalasin B nor colchicine inhibited 3H-threonine or 3H-glucosamine incorporation into mucous and serous cells of the submucosal glands or goblet cells of the airway epithelium. Colchicine (10−5 M) but not cytochalasin B significantly reduced the rate at which labeled macromolecules were transported through mucous, serous and goblet cells but this effect was not observed until 4 h after the addition of colchicine. Neither cytochalasin B nor colchicine affected the basal rate of labeled-macromolecule discharge from mucous, serous or goblet cells. At a concentration of 10−5 M, both agents completely inhibited the increase in labeled-macromolecule discharge induced in mucous and serous cells by methacholine.
Our results suggest that in the submucosal gland of human airways microtubules and microfilaments may be important in secretagogue-induced but not in baseline cellular glycoconjugate discharge, implying that the mechanisms of the two processes differ significantly. Furthermore, a role for microtubules is suggested in the transport of secretory granules through mucous, serous and goblet cells.
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References
Adler KB, Craighead JE (1978) Function of microtubules and microfilaments in the secretion of mucin by porcine tracheobronchial tissue in organ culture. Fed Proc 37:266
Allison AC, Davies P (1974) In: Ceccarelli B, Clementi F, Meldolesi J (eds) Interaction of membranes, microfilaments and microtubules in endocytosis and exocytosis. Advances in Cytopharmacology, Vol II, New York, pp 237–248
Bauduin HC, Stock C, Vincent D, Grenier JF (1975) Microfilamentous system and secretion of enzyme in the exocrine pancreas. Effect of cytochalasin B. J Cell Biol 66:165–181
Butcher FR, Goldman RH (1972) Effect of cytochalasin B and colchicine on the stimulation of α-amylase release from rat parotid tissue slices. Biochem Biophys Res Commun 48:23–29
Chakrin LW, Baker AP, Christian P, Wardell JR Jr (1973) Effect of cholinergic stimulation on the release of macromolecules by canine trachea in vitro. Am Rev Respir Dis 108:69–76
Coles SJ (1976) Regulation of the secretion cycles of mucous and serous cells in the human bronchial gland. In: Elstein M, Parke DV (eds) Mucus in Health and Disease. Plenum Press, New York London, p 155
Coles SJ, Reid L (1978) Glycoprotein secretion in vitro by human airway: Normal and chronic bronchitis. Exp Mol Pathol 29:326–341
Gallagher JT, Kent PW, Passatore M, Phipps RJ and Richardson PS (1975) The composition of tracheal mucus and the nervous control of its secretion in the cat. Proc R Soc Lond B 192:49–76
Gillespie E, Levine RJ, Malawista SE (1968) Histamine release from rat peritoneal mast cells: inhibition by colchicine and potentiation by deuterium oxide. J Pharmacol Exp ther 164:158–165
Kletzien RF, Perdue JF, Springer A (1972) Cytochalasin A and B. Inhibition of sugar uptake in cultured cells. J Biol Chem 247:2964–2966
Lacy PE, Howell SL, Young DA, Fink CJ (1968) New hypothesis of insulin secretion. Nature 219:1177–1179
Last JA, Jennings MD, Schwartz LW, Cross CE (1977) Glycoprotein secretion by tracheal explants cultured from rats exposed to ozone. Am Rev Respir Dis 116:695–703
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin-phenol reagent. J Biol Chem 193:265–275
Meyrick B, Reid L (1970) Ultrastructure of cells in the human bronchial submucosal glands. J Anat 107:281–299
Meyrick B, Reid L (1975) In vitro incorporation of [3H] threonine and [3H] glucose by the mucous and serous cells of the human bronchial submucosal gland. J Cell Biol 67:320–344
Mizel SB, Wilson L (1972) Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B. J Biol Chem 247:4102–4105
Nemeth EF, Douglas WW (1978) Effects of microfilament-active drugs, phalloidin and the cytochalasins A and B, on exocytosis in mast cells evoked by 48/80 or A 23187. Naunyn-Schmiedeberg's Arch Pharmacol 302:153–163
Neutra MR, Grand JF, Trier JS (1977) Glycoprotein synthesis, transport and secretion by epithelial cells of human rectal mucosa. Normal and cystic fibrosis. Lab Invest 36:535–546
Patzelt C, Brown D, Jeanrenaud B (1977) Inhibitory effect of colchicine on amylase secretion by rat parotid glands. Possible localization in the Golgi area. J Cell Biol 73:578–593
Rogers AR (1973) Techniques of Autoradiography. Elsevier, Amsterdam London New York, p 89
Schofield JG (1971) Cytochalasin B and release of growth hormone. Nature 234:215–216
Schroeder TE (1970) The contractile ring I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B. Z Zellforsch 109:431–449
Stock C, Launay JF, Grenier JF, Bauduin H (1978) Pancreatic acinar cell changes induced by caerulein, vinblastine, deuterium oxide and cytochalasin B in vitro. Lab Invest 38:157–164
Sturgess J, Reid L (1972) Secretory activity of the human bronchial mucous glands in vitro. Exp Mol Pathol 16:362–381
Wessels NK, Spooner BS, Ash JF, Bradley MO, Luduena MA, Taylor EL, Wrenn JT, Yamada KM (1971) Microfilaments in cellular and developmental processes. Science 171:135–143
Williams JA (1977) Effects of cytochalasin B and pancreatic acinar cell structure and secretion. Cell Tissue Res 179:453–466
Williams JA, Lee M (1976) Microtubules and pancreatic amylase release by mouse pancreas in vitro. J Cell Biol 71:795–806
Williams JA, Wolff J (1971) Cytochalasin B inhibits thyroid secretion. Biochem Biophys Res Commun 44:422–425
Wilson L, Bamburg JR, Mizel SB, Grisham LM, Cresswell KM (1974) Interaction of drugs with microtubule proteins. Fed Proc 33:158–166
Yeager H, Massaro G, Massaro D (1971) Glycoprotein synthesis by the trachea. Am Rev Respir Dis 103:188–197
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Supported by National Institutes of Health Research Grant 5R01HL22444. The authors gratefully acknowledge the technical assistance of Mr. Tudor Williams, Mr. Eduardo Quintanilla and Ms. Maureen Hayes
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Coles, S.J., Reid, L. Inhibition of glycoconjugate secretion by colchicine and cytochalasin B. Cell Tissue Res. 214, 107–118 (1981). https://doi.org/10.1007/BF00235149
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DOI: https://doi.org/10.1007/BF00235149