Abstract
Hairy root cultures were induced from leaf explants of Linum flavum by infection with Agrobacterium rhizogenes. The transformed nature of tissue was confirmed by the production of opines. The cultures produced 1.5 to 3.5% of the lignan 5-methoxypodophyllotoxin (5-MPT) on a dry weight basis, which was 2 to 5 times higher than the 5-MPT content in untransformed root cultures and 5 to 12 times higher than in L. flavum cell suspensions. The 5-MPT production as a function of time was up to four times higher than that in cell suspensions.
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Abbreviations
- NAA:
-
1-naphthaleneacetic acid
- BAP:
-
6-benzylaminopurine
- 5-MPT:
-
5-methoxypodophyllotoxin
- DW:
-
dry weight
- GC-MS:
-
gas-chromatography coupled electron impact mass spectrometry
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Communicated by K. Hahlbrock
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Oostdam, A., Mol, J.N.M. & van der Plas, L.H.W. Establishment of hairy root cultures of Linum flavum producing the lignan 5-methoxypodophyllotoxin. Plant Cell Reports 12, 474–477 (1993). https://doi.org/10.1007/BF00234715
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DOI: https://doi.org/10.1007/BF00234715