Abstract
A microprojection protocol using the DuPont Biolistic™ particle delivery system and the β-glucuronidase (GUS) reporter gene fused with the 35S promoter of Cauliflower mosaic virus (CaMV) was developed for Picea mariana callus. Comparison of four tungsten microprojectile sizes showed the highest transient gene expression with 1.11μm diameter particles. Adsorption of DNA on the microcarriers using calcium chloride led to higher GUS gene activity than using polyethylene glycol. GUS gene activity in P. mariana was the highest when cells were treated 5 and 6 days after subculturing to fresh media. The wheat ABA-inducible Em gene promoter yielded 4.5 times higher GUS gene activity than the 35S CaMV promoter. Comparison of transient GUS gene expression among 10 P. mariana embryogenic cell lines from six different open-pollinated families showed comparable gene activity, with the exception of one family showing no GUS gene activity.
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Attree SM, Dunstan DI, Fowke LC (1989) Biotechnology 7:1060–1062.
Bekkaoui F, Dunstan DI (1989) Can J For Res 19:1316–1321.
Bekkaoui F, Datla RSS, Pilon M, Tautorus TE, Crosby WL, Dunstan DI (1990) Theor Appl Genet 79:353–359.
Charest PJ, Devantier Y, Ward C, Jones C, Schaffer U, Klimaszewska K (1991) Can J Bot (in press).
Cheliak WM, Klimaszewska K (1991) Theor Appl Genet (in press).
Cheliak WM, Rogers DL (1990) Can J For Res 20:452–463
Durzan DJ, Gupta PK (1987) Plant Sci 52:229–235.
Ellis DD, McCabe D, Russel D, McCown BH, Martinell B (1991) Plant Mol. Biol. (in press).
Gupta PK, Durzan DJ (1986) In vitro Cell Dev Biol 22s:685–688.
Hakman I, von Arnold S (1985) J. PLant. Physiol 121:149–158.
Jefferson RA, Kavanagh TA, Bevan MW (1987) Embo J 6:3901–3907.
Kawata T, Nakayama T, Ohtsubon, Tabata T, Iwabuchi M (1990) Dev Gen 11:205–213.
Klein TM, Wolf ED, Wu R, Sandford JC (1987) Nature 327:70–73.
Klimaszewska K (1989) Plant Sci 63: 95–103.
Maliga PA, Breznovits SZ, Marton L (1973) Nature New Biol 244:29–30.
Marcotte WR, Bayley CC, Quatrano RS (1988) Nature 335:454–457.
Murashige T, Skoog F (1962) Physiol Plant 15:473–497.
Nagata T, Okada K, Kawazu T, Takebe I (1987) Mol Gen Genet 207:242–244.
Sanford JC (1990) Physiol Plantarum 79:206–209.
Schuller A, Reuther G, Geier T (1989) Plant Cell Tissue Organ Culture 17:53–58.
Sederoff RR, Stomp AM (1990) DNA transfer in conifers. In: Libby WJ, Ahuja MR (eds.) Clonal forestry: genetics, biotechnology and application. Springer-Verlag (In press).
Stomp AM, Weissinger AK, Sederoff RR (1991) Plant Cell Reports (In press).
Tartof KD, Hobbs CA (1987) Focus 9:2.
Tautorus TE, Bekkaoui F, Pilon M, Datla RSS, Crosby WL, Fowke LC, Dunstan DI (1989) Theor Appli Genet 78:531–536
Topfer R, Schell J, Steinbiss HH (1988) Nucl Acids Res 16:8725.
von Aderkas P, Klimaszewska K, Bonga JM (1990) Can J For Res 20:9–14.
Wilson SM, Thorpe TA, Moloney MM (1989) Plant Cell Reports 7:704–707.
Yibrah HS, Clapham DH (1990) In IUFRO Molecular genetics working party S.04.06. Stanford Sierra Camp, Fallen Leaf Lake, Lake Tahoe, CA.. Sept 30–Oct 4, 1990.
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Duchesne, L.C., Charest, P.J. Transient expression of the β-glucuronidase gene in embryogenic callus of Picea mariana following microprojection. Plant Cell Reports 10, 191–194 (1991). https://doi.org/10.1007/BF00234293
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DOI: https://doi.org/10.1007/BF00234293