Summary
Sunflower hypocotyl protoplasts (Helianthus annuus L.) from 5 PIONEER genotypes (PT024, SMF3, EMIL, HA300*PT024, VK5F) and 1 public line (RHa 274) formed colonies at frequencies of up to 60% when plated in 0.25ml agarose beads in a modified L4 medium (Lenée and Chupeau 1986) containing 3mg/l NAA, 1mg/l BA and 0.1mg/l 2,4-D, and 1000mg/l casamino acids. Protoplast-derived colonies grew slowly into calli. Organogenesis was obtained from callus of PT024 on a MS medium containing NAA and BA at 1mg/l and GA at 0.1mg/l. Freshly excised shoots were induced to root by an IAA treatment. Regenerated plants were transferred to the greenhouse and seed was harvested within 7 months of the initial protoplast isolation.
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Abbreviations
- BA:
-
6-benzylaminopurine
- NAA:
-
α-naphtaleneacetic acid
- GA:
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS:
-
Murashige and Skoog mineral elements
- B5:
-
Gamborg mineral elements
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Burrus, M., Chanabe, C., Alibert, G. et al. Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.). Plant Cell Reports 10, 161–166 (1991). https://doi.org/10.1007/BF00234286
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DOI: https://doi.org/10.1007/BF00234286