Abstract
Somatic embryos of Asparagus cooperi were encapsulated as single embryos approximately 4–6 mm in diameter to produce individual synthetic seeds. The frequency of conversion of artificial seeds to plants was 34%. This frequency was affected by the concentration of calcium chloride, the commercial sources of sodium alginate, and the nutrient medium. The conversion frequency of artificial seeds to seedling plants was 8.3% after storage for 90 days at 2°C.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- NAA:
-
α-Naphthaleneacetic acid
- ABA:
-
Abscisic acid
- GA3 :
-
Gibberellic acid
- Kn:
-
6-Furfurylaminopurine
- MS:
-
Murashige and Skoog (1962) medium
- SH:
-
Schenk and Hildebrandt (1972) medium
- WM:
-
White (1963) medium
References
Bapat, V. A. and Rao, P. S. 1988. Plant Cell Rep. 7: 434–436
Dutta, S. K. and Potrykus, I. 1989. Theor. Appl. Genet. 77: 820–824.
Ghosh, B. and Sen, S. 1989. Curr. Sci. 58: 256–257.
Ghosh, B. and Sen, S. 1991. Plant Cell Rep. 9: 667–670.
Ghosh, B. and Sen, S. 1992a. Plant Science 82: 119–124.
Ghosh, B. and Sen, S. 1992b. Plant Science (Communicated).
Kar, D. K. and Sen, S. 1986. Int. J. Crude Drug Res. 24: 131–133.
Lakshmana Rao, P. V. and Singh, B. 1991. Plant Cell Rep. 10: 7–11.
Murashige, T. and Skoog, F. 1962. Physiol Plant 15: 473–497.
Redenbaugh, K., Paasch, B. D., Nichol, J. W., Kossler, M. E., Viss, P. R. and Walker A. W. 1986 Bio/Technology 4: 797–801.
Redenbaugh, K., Slade, D., Viss. P. and Fujii, J. 1987. Hort Science 22: 803–809.
Redenbaugh, K., Fujii, J., Slade, D., Viss, P. and Kossler, M. 1991. Artificial seeds-encapsulated somatic embryos. In: Biotechnology in Agriculture and Forestry 17 (High tech and Microprogation I) (Ed. YPS Bajaj) Springer-Verlag, Berlin, pp. 395–416.
Schenk, R. U. and Hildebrandt, A. C. 1972. Can. J. Bot. 50: 199–204.
White, P. R. 1963. In: The cultivation of animal and plant cells. The Ronald press, NY, 228.
Author information
Authors and Affiliations
Additional information
Communicated by J. J. Finer
Rights and permissions
About this article
Cite this article
Ghosh, B., Sen, S. Plant regeneration from alginate encapsulated somatic embryos of Asparagus cooperi baker. Plant Cell Reports 13, 381–385 (1994). https://doi.org/10.1007/BF00234142
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00234142