Summary
Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20–60% of the protoplasts emitted an intense green light when illuminated with blue (450–490 nm) light.
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Abbreviations
- GUS:
-
β-glucuronidase
- LUC:
-
luciferase
- NPTII:
-
neomycinphosphotransferase
- CaMV:
-
cauliflower mosaic virus
- MUG:
-
4-methylumbelliferyl β-D-glucuronide
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Communicated by W. Parrott
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Niedz, R.P., Sussman, M.R. & Satterlee, J.S. Green fluorescent protein: an in vivo reporter of plant gene expression. Plant Cell Reports 14, 403–406 (1995). https://doi.org/10.1007/BF00234043
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DOI: https://doi.org/10.1007/BF00234043