Abstract
The subcommissural organ is an ependymal brain gland that secretes glycoproteins to the cerebrospinal fluid (CSF) of the thrid ventricle. They condense to form a fibre, Reissner's fibre (RF), that runs along the aqueduct and fourth ventricle and the central canal of the spinal cord. A single injection of an antibody against the secretory glycoproteins of RF into a lateral ventricle of adult rats results in animals permanently deprived of RF in the central canal and bearing a “short” RF extending only along the aqueduct and the fourth ventricle. These animals, together with untreated control animals were used to investigate the probable influence of RF in the circulation of CSF in the central canal of the spinal cord. For this purpose, two tracers (horseradish peroxidase and rabbit immunoglobulin) were injected into the ventricular CSF. The animals were killed 13, 20, 60, 120 and 240 min after the injection, and the amount of the tracers was estimated in tissue sections obtained at proximal, medial and distal levels of the spinal cord. In rats deprived of RF, a significant decrease in the amount of tracers present in the central canal was observed at all experimental intervals, being more evident at 20 min after the injection of the tracers. This suggests that lacking a RF in the central canal decreases the bulk flow of CSF along the central canal. Turbulences of the CSF at the entrance of the central canal of RF-deprived rats might explain the inability of the regenerating RF to progress along the central canal, as well as the reduced flow of CSF in the central canal of these animals.
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References
Afzelius BA (1985) The immotile-cilia syndrome: a microtubule-associated defect. CRC Crit Rev Biochem 19 (1):63–87
Braathen LR, Forre OT, Husby G, Williams RC (1979) Evidence for Fc IgG receptors and complement factor C3b receptors in human choroid plexus. Clin Immunol Immunopathol 14:284–291
Bradbury MWB, Lathem W (1965) A flow of cerebrospinal fluid along the central canal of the spinal cord of the rabbit and communications between this canal and the sacral subarachnoid space. J Physiol (Lond) 181:785–800
Brightman MW (1965) The distribution within the brain of ferritin injected into cerebrospinal fluid compartments. I. Ependymal distribution. J Cell Biol 26:99–123
Cifuentes M, Fernández-Llebrez P, Pérez J, Pérez-Fígares JM, Rodríguez EM (1992) Distribution of intraventricularly injected horseradish peroxidase in cerebrospinal fluid compartments of the rat spinal cord. Cell Tissue Res 270:485–494
Davson H, Welch K, Segal MB (1987) The physiology and pathophysiology of the cerebrospinal fluid. Churchill Livingstone, New York
Ermisch H (1973) Zur Charakterisierung des Komplexes Subkommissuralorgan-Reissnerscher Faden und seiner Beziehung zum Liquor unter besonderer Berücksichtigung autoradiographischer Untersuchungen sowie funktioneller Aspekte. Wiss Z Karl Marx Univ Leipzig Mat Naturwiss R 22:297–336
Graham RC, Karnovsky M (1966) The early stages of absorption of injected horseradish peroxidase in the proxumal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Greenstone MA, Jones RWA, Dewar A, Neville BGR, Cole PJ (1984) Hydrocephalus and primary ciliary dyskinesia. Arch Dis Child 59:481–482
Herrera H (1988) Procesamiento del material secretorio del órgano subcomisural. Doctoral Thesis, Universidad Austral de Chile, Valdivia, Chile
Hill JR (1957) The influence of drugs on ciliary activity. J Physiol (Lond) 139:157–166
Hofer H, Meinel W, Erhardt H, Wolter A (1984) Preliminary electron-microscopical observations on the ampulla caudalis and the discharge of the material of Reissner's fiber into the capillary system of the terminal part of the tail of Ammocoetes (Agnathi). Gegenbaurs Morphol Jahrb 130:77–110
Irigoin C, Rodríguez EM, Heinrichs M, Frese K, Herzog S, Oksche A, Rott R (1990) Immunocytochemical study of the subcommissural organ of rats with postnatal induced hydrocephalus. Exp Brain Res 82:384–392
Jones HC, Bucknall RM (1988) Inherited prenatal hydrocephalus in the H-Tx rat: a morphological study. Neuropathol Appl Neurobiol 14:263–274
Krisch B, Leonhardt H, Oksche A (1984) Compartments and perivascular arrangement of the meninges covering the cerebral cortex of the rat. Cell Tissue Res 238:459–474
Milhorat TH (1975) The third circulation revisited. J Neurosurg 42:628–645
Nakayama Y (1976) The openings of the central canal in the filum terminale internum of some mammals. J Neurocytol 5:531–544
Nelson DJ, Wright EM (1974) The distribution, activity, and function of the cilia in the frog brain. J Physiol (Lond) 243:63–78
Newberne PM (1962) The subcommissural organ of the vitamin B12 deficient rat. J Nutr 76:393–414
Nualart F, Hein S, Rodríguez EM, Oksche A (1991) Identification and partial characterization of the secretory glycoproteins of the bovine subcommissural organ-Reissner's fiber complex. Evidence for the existence of two precursor forms. Mol Brain Res 11:227–238
Oksche A (1961) Vergleichende Untersuchungen über sekretorische Aktivität der Subkommissuralorgans und den Gliacharakter seiner Zellen. Z Zellforsch 54:549–612
Olsson R (1958) Studies on the subcommisural organ. Acta Zool (Stockh) 39:71–102
Overholser MD, Whitley JR, O'Dell BL, Hogan AG (1954) The ventricular system in hydrocephalic rat brains produced by a deficiency of vitamin B12 and folic acid in the maternal diet. Anat Rec 120:917–934
Peruzzo B, Rodríguez S, Delannoy L, Hein S, Rodríguez EM (1987) Ultrastructural immunocytochemical study of the massa caudalis of the subcommissural organ-Reissner's fiber complex in lamprey larvae (Geotria australis): evidence for a terminal vascular route of secretory material. Cell Tissue Res 247:367–376
Reissner E (1860) Beiträge Zur Kenntnis vom Bau des Rückenmarks von Petromyzon fluviatilis L. Arch Anat Physiol 77:545–588
Rodríguez EM, Oksche A (1993) Historical landmarks in the investigation of the subcommissural organ and Reissner's fiber. In: Rodríguez EM, Oksche A, Fernández-Llebrez P (eds) The subcommissural organ. An ependymal brain gland. Springer, Berlin Heidelberg New York, pp 9–18
Rodríguez EM, Oksche A, Hein S, Rodríguez S, Yulis CR (1984) Comparative immunocytochemical study of the subcommissural organ. Cell Tissue Res 237:427–441
Rodríguez EM, Herrera H, Peruzzo B, Rodríguez S, Hein S, Oksche A (1986) Light-and electron-microscopic lectin histochemistry of the subcommissural organ: evidence for processing of the secretory material. Cell Tissue Res 243:545–559
Rodríguez EM, Rodríguez S, Schoebitz K, Yulis CR, Hoffmann P, Manns V, Oksche A (1989) Light-and electron-microscopic investigation of the rat subcommissural organ grafted under the kidney capsule, with particular reference to immunocytochemistry and lectin histochemistry. Cell Tissue Res 258:499–514
Rodríguez EM, Oksche A, Rodríguez S, Nualart F, Irigoin C, Fernández-Llebrez P, Pérez-Figares JM (1990) Subcommissural organ and cerebrospinal fluid. A biochemical and experimental study. In: Epple A (ed) Progress in comparative endocrinology. Wiley-Liss, New York, pp 282–292
Rodríguez EM, Oksche A, Hein S, Yulis CR (1992) Cell biology of the subcommissural organ. Int Rev Cytol 135:39–121
Rodríguez S, Rodríguez PA, Banse C, Rodríguez EM, Oksche A (1987) Reissner's fiber, massa caudalis and ampulla caudalis in the spinal cord of lamprey larvae (Geotria ausytalis). Cell Tissue Res 247:359–366
Rodríguez S, Rodríguez EM, Jara P, Peruzzo B, Oksche A (1990) Single injection into the cerebrospinal fluid of antibodies against the secretory material of the subcommissural organ reversibly blocks formation of the Reissner's fiber: immunocytochemical investigation in the rat. Exp Brain Res 81:113–124
Rodríguez S, Quiñones L, Rodríguez E (1993) Immunological blockade of the subcommissural organ-Reissner's fiber complex. In: Rodríguez EM, Oksche A, Fernández-Llebrez P (eds) The subcommissural organ. An ependymal brain gland. Springer, Berlin Heidelberg New York, pp 279–288
Sternberger LA, Hardy PH, Cuculis JJ Jr, Meyer HG (1970) The unlabeled antibody enzyme method of immunocytochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antiperoxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Shimizu A, Koto M (1992) Ultrastructure and movement of the ependymal and tracheal cilia in congenitally hydrocephalic WIC-Hyd rats. Child's Nerv Syst 8:25–32
Takeuchi IK, Takeuchi YK (1986) Congenital hydrocephalus following X-irradiation of pregnant rats on an early gestational day. Neurobehav Toxicol Teratol 8:143–150
Takeuchi IK, Kimura R, Matsuda M, Shoji R (1987) Absence of subcommissural organ in the cerebral aqueduct of congenital hydrocephalus spontaneously occurring in MT/Hokldr mice. Acta Neuropathol 73:320–322
Takeuchi IK, Kimura R, Shoji R (1988) Dysplasia of subcommissural organ in congenital hydrocephalus spontaneously occurring in CWS/Idr rats. Experientia 44:338–340
Warr WB, DeOlmos JS, Heimer L (1981) Horseradish peroxidase. The basic procedure. In: Heimer L, RoBard MT (eds) Neuroanatomical tract-tracing methods. Plenum, New York, pp 207–262
Wagner HJ, Pilgrim Ch, Brandi J (1974) Penetration and removal of horseradish peroxidase injected into the cerebrospinal fluid: role of cerebral perivascular spaces, endothelium and microglia. Acta Neuropathol 27:299–315
Wood JH (1983) Physiology, pharmacology and dynamics of cerebrospinal fluid. In: Wood JK (ed) Neurobiology of cerebrospinal fluids. Plenum, New York, pp 1–16
Worthington WC, Cathcart RSIII (1966) Ciliary currents on ependymal surfaces. Ann NY Acad Sci 130:944–950
Wright EM (1978) Transport processes in the formation of the cerebrospinal fluid. Rev Physiol Biochem Pharmacol 83:1–34
Wright EM (1982) Secretion and circulation of the cerebrospinal fluid. In: Rodríguez EM, Greidanus TjB van (eds) Cerebrospinal fluid (CSF) and peptide hormones. Karger, Basel, pp 4–14
Yamadori T, Nara K (1979) The direction of ciliary beat on the wall of the lateral ventricle and the currents of the cerebrospinal fluid in the brain ventricles. Scanning Electron Microsc 3:335–340
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Cifuentes, M., Rodríguez, S., Pérez, J. et al. Decreased cerebrospinal fluid flow through the central canal of the spinal cord of rats immunologically deprived of Reissner's fibre. Exp Brain Res 98, 431–440 (1994). https://doi.org/10.1007/BF00233981
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DOI: https://doi.org/10.1007/BF00233981