Summary
A dramatic increase in rosmarinic acid (RA) content in cultured cells of Lithospermum erythrorhizon was observed after their exposure to methyl jasmonate (MJ). Preceding the induced RA accumulation, phenylalanine ammonia-lyase (PAL) and 4-hydroxyphenylpyruvate reductase (HPR) activities increased rapidly and transiently, whereas tyrosine aminotransferase (TAT) activity showed only a slight increase. The elicitation activity of MJ was much higher than that of yeast extract (YE) in terms of the induction of PAL and HPR activities, RA accumulation and incorporation of both 14C-phenylalanine and 14C-tyrosine into RA. However, the response of the cultured cells to MJ-treatment was slower than that to YE-treatment.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- LS:
-
Linsmaier and Skoog
- HPR:
-
4-hydroxyphenylpyruvate reductase
- PAL:
-
phenylalanine ammonia-lyase
- TAT:
-
tyrosine aminotransferase
- MJ:
-
methyl jasmonate
- YE:
-
yeast extract
References
Bradford MM (1976) Anal. Biochem. 72: 248–254
De-Eknamukul W, Ellis BE (1987) Phytochemistry 30: 2877–2881
Ellis BE, Towers GHN (1970) Biochem. J. 118: 287–291
Farmer EE, Ryan CA (1990) Proc. Natl. Acad. Sci. USA 87: 7713–7716
Franceschi VR, Grimes HD (1991) Proc. Natl. Acad. Sci. USA 88: 6745–6749
Gundlach H, Müller MJ, Kutchan TM, Zenk MH (1992) Proc. Natl. Acad. Sci. USA 89: 2389–2393
Häusler E, Petersen M, Alfermann AW (1991) Z. Naturforsch. 46C: 371–376
Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127
Mizukami H, Ellis BE (1991) Plant Cell Rep. 10: 321–324
Mizukami H, Ogawa T, Ohashi H, Ellis BE (1992) Plant Cell Rep. 11: 480–483
Nojiri H, Yamane H, Seto H, Yamaguchi I, Murofushi N, Yoshihara T, Shibaoka H (1992) Plant Cell Physiol. 33: 1225–1231
Petersen MS (1991) Phytochemistry 30: 2877–2881
Petersen M, Dombrowski K, Gertlowski C, Häusler E, Karwatzki B, Meihard J, Alfermann AW (1992) In: Oono K, Hirabayashi T, Kikuchi S, Handa H, Kajiwara K (ed.) Plant tissue culture and gene manipulation for breeding and formation of phytochemicals, National Institute of Agrobiological Resources, Tsukuba, pp. 297–310
Staswick PE (1992) Plant Physiol. 99: 804–807
Sumaryono W, Proksch P, Hartmann T, Nimitz M, Wray V (1991) Phytochemistry 30: 3267–3271
Weiler EW, Albrecht T, Groth B, Xia Z-Q, Luxem M, Liss H, Andert L, Spengler P (1993) Phytochemistry 32: 591–600
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Mizukami, H., Tabira, Y. & Ellis, B.E. Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Reports 12, 706–709 (1993). https://doi.org/10.1007/BF00233424
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DOI: https://doi.org/10.1007/BF00233424