Abstract
Protoplast fusion using polyethylene glycol (PEG) was conducted to combine Citrus sinensis (L.) Osbeck cv. ‘Hamlin’ sweet orange protoplasts, isolated from nucellus-derived embryogenic callus with Atalantia ceylanica (Arn.) Oliv, leaf protoplasts. Five plants regenerated from independent fusion events following protoplast culture were identified as intergeneric allotetraploid somatic hybrids of ‘Hamlin’ sweet orange and A. ceylanica, and confirmed by isozyme analysis and chromosome number determination in root tip cells (2n=4x=36). Two different types of leaf morphology were observed among the hybrids (normal and narrow), although no differences in chromosome number nor isozyme banding patterns were observed. This is the first report of the production of hybrid plants between these sexually incompatible genera.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bitters WP, Brusca JA, Cole DA (1964) Calif Citrog 49: 443–448
Bitters WP, Cole DA, McCarty CD (1977) Proc Int Soc Citriculture 2: 561–567
Cardy BJ, Stuber CW, Goodman MM (1981) Inst Statist Mimeo Serv, No 1317, North Carolina State Univ, Raleigh
Gmitter FG, Jr, Ling XB, Deng XX (1990) Theor Appl Genet 80: 785–790
Grosser JW, Chandler JL (1987) Scientia Hort 31: 253–257
Grosser JW, Gmitter FG, Jr (1990a) Plant Breed Rev 8: 339–374
Grosser JW, Gmitter FG, Jr (1990b) HortScience 25: 147–151
Grosser JW Gmitter FG, Jr (1990c) In: Bennett AB, O'Neill SD (eds), Horticultural biotechnology, plant biology, Vol 25, Wiley-Liss, New York, pp 31–41
Grosser JW, Gmitter FG, Jr, Louzada ES, Chandler JL (1992a) HortScience 27: 1125–1127
Grosser JW, Gmitter FG, Jr, Sesto F, Deng XX, Chandler JL (1992b) J Am Soc Hort Sci 117: 169–173
Grosser JW, Gmitter FG, Jr, Chandler JL (1988) Theor Appl Genet 75: 397–401
Grosser JW, Gmitter FG, Jr, Tusa N, Chandler JL (1990) Plant Cell Rpt 8: 656–659
Iwamasa M, Nito N, Ling JT (1988) In: Goren R, Mendel K (eds), Proc Sixth Int Citrus Cong, Balaban Publisher, Margraf Publisher, Middle-East, pp 123–130
Kobayashi S, Ohgawara T, Fugiwara K, Oiyama, I (1991) Theor Appl Genet 82: 6–10
Louzada ES, Grosser JW, Gmitter FG, Jr, Deng XX, Tusa N, Nielsen B, Chandler JL (1992) HortScience 27: 1033–1036
Sabbah SM, Grosser JW, Chandler JL, Louzada ES (1991) Proc Fla State Hort Soc 104: 188–191
Tusa N, Grosser JW, Gmitter FG, Jr (1990) J Am Soc Hort Sci 115: 1043–1046
Vallejos CE (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetic and breeding, Part A, Elsevier, Amsterdam, pp 469–516
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Florida Agricultural Experiment Station Journal Series No. R-03069.
Rights and permissions
About this article
Cite this article
Louzada, E.S., Grosser, J.W. & Gmitter, F.G. Intergeneric somatic hybridization of sexually incompatible parents: Citrus sinensis and Atalantia ceylanica . Plant Cell Reports 12, 687–690 (1993). https://doi.org/10.1007/BF00233420
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00233420