Summary
Transgenic cucumber (Cucumis sativus L.) plants were successfully obtained from hypocotyl explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid with NOS-nptII, CaMV 35S-I-gus and CaMV 35S-hph genes. Acetosyringone enhanced the efficiency of transformation at the cut surface cells of hypocotyl explants during five days of co-cultivation. Transformed cells were more effectively selected using 20–30 mg/l hygromycin B than using 50–100 mg/l kanamycin. Shoot regeneration occurred within 4–6 wks, and 12 of 21 regenerated plantlets displayed strong GUS expression in the very young leaves. All of 8 GUS-positive R0 plants examined showed single or a few positive bands by Southern blot analysis. The expression of the CaMV 35S-I-gus gene was observed in various tissues and organs of R0 and R1 transgenic cucumber plants.
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Akama K, Shiraishi H, Ohta S, Nakamura K, Okada K, Shimura Y (1992) Plant Cell Reports 12: 7–11
Beachy RN, Loesch-Fries S, Tumer NE (1990) Annu. Rev. Phytopathol. 28: 451–474
Benfey PN, Ren L, Chua NH (1989) EMVO J. 8: 2195–2202
Chee PP (1990) Plant Cell Reports 9: 245–248
Dong JZ, Yang MZ, Jia SR, Chua NH (1991) Bio/Technology 9: 858–863
Hood EE, Helmer GL, Fraley RT, Chilton MD (1986) J. Bacteriology 168: 1291–1301
Jefferson RA, Kavanagh TA, Bevan MW (1987) EMBO Journal 6: 3901–3907
Lal R and Lal S (1993) Genetic Engineering of Plants for Crop Improvement, CRC Press, Boca Raton, Ann Arbor, London, Tokyo.
Ohta S, Mita S, Hattori T, Nakamura K (1990) Plant Cell Physiol 31: 805–813
Richards E. (1987) In Current Protocols in Molecular Biology, Unit 2. 3. ed. by Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K, John Wiley & Sons, New York.
Sarmento GG, Alpert K, Tang FA, Punja ZK (1992) Plant Cell, Tissue and Organ Culture 31: 185–193
Terada R, Shimamoto K (1990) Mol Gen Genet 220: 389–392
Trulson AJ, Simpson RB, Shahin EA (1986) Theor Appl Genet 73: 11–15
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Communicated by K. Shimamoto
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Nishibayashi, S., Kaneko, H. & Hayakawa, T. Transformation of cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants. Plant Cell Reports 15, 809–814 (1996). https://doi.org/10.1007/BF00233145
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DOI: https://doi.org/10.1007/BF00233145