Summary
Restriction fragment length polymorphisms (RFLPs) have been used to detect chloroplast (cp) and mitochondrial (mt) DNA variation among 18 apple cultivars and three rootstocks. The distribution of RFLP patterns allowed the assignment of these genotypes into three groups of cytoplasmic relatedness. Our results also demonstrate maternal inheritance of cp- and mtDNAs in apple. Thus, the organelle DNA assay provides a convenient and reliable method to assess cytoplasmic diversity within the apple germ-plasm collection and to trace the maternal lineages involved in the evolution of apple.
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References
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Fragoso LL, Nichols SE, Levings CS III (1989) Rearrangements in maize mitochondrial genes. Genome 31:160–168
Gracen PE, Grogan CO (1974) Diversity and suitability for hybrid production of different sources of cytoplasmic male sterility in maize. Agron J 66:654–657
Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: Lessons from cytoplasmic-nuclear interactions affecting male fertility in plants. Int Rev Cytol 94:213–267
Huh TY, Gray MW (1982) Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms. Plant Mol Biol 1:245–249
Leaver CJ, Gray MW (1982) Mitochondrial genome organization and expression in higher plants. Annu Rev Plant Physiol 33:373–402
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Marechal L, Runeberg-Roos P, Grienenberger JM, Colin J, Weil JH, Lejeune B, Quetier F, Lonsdale DM (1987) Homology in the region containing a tRNATrp gene and a (complete or partial) tRNAPro gene in wheat mitochondrial and chloroplast genomes. Curr Genet 12:91–98
Mikami T, Sugiura M, Kinoshita T (1984) Molecular heterogeneity in mitochondrial and chloroplast DNAs from normal and male sterile cytoplasms in sugar beets. Curr Genet 8:319–322
Morikami A, Nakamura K (1987a) Structure and expression of pea mitochondrial F1 ATPase α-subunit gene and its pseudogene involved in homologous recombination. J Biochem 101:967–976
Morikami A, Nakamura K (1987b) The pea mitochondrial AT-Pase subunit 9 gene is located upstream of the ATPase α-subunit gene. Nucleic Acids Res 15:4692
Palmer JD (1987) Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Am Nat 130:S6-S29
Pring DR, Lonsdale DM (1985) Molecular biology of higher plant mitochondrial DNA. Int Rev Cytol 97:1–46
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Schuster W, Brennicke A (1988) Interorganellar sequence transfer: Plant mitochondrial DNA is nuclear, is plastid, is mitochondrial. Plant Sci 54:1–10
Sears BB (1980) Elimination of plastids during spermatogenesis and fertilization in the plant kingdom. Plasmid 4:233–255
Senda M, Harada T, Mikami T, Sugiura M, Kinoshita T (1991) Genomic organization and sequence analysis of the cytochrome oxidase subunit II gene from normal and male-sterile mitochondria in sugar beet. Curr Genet 19:175–181
Stern DB, Lonsdale DM (1982) Mitochondrial and chloroplast genomes of maize have a 12 kb DNA sequence in common. Nature 299:698–702
Sugiura M, Kusuda J (1979) Molecular cloning of tobacco chloroplast ribosomal RNA genes. Mol Gen Genet 172:137–141
Tsunewaki K (1989) Plasmon diversity in Triticum and Aegilops and its implication in wheat evolution. Genome 31:143–154
Vinterhalter DV, James DJ (1986) The use of peroxidase polymorphism in the identification of Malling and Malling Merton apple rootstocks. J Hortic Sci 61:147–152
Whatley JM (1982) Ultrastructure of plastid inheritance: green algae to angiosperms. Biol Rev 57:527–569
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Ishikawa, S., Kato, S., Imakawa, S. et al. Organelle DNA polymorphism in apple cultivars and rootstocks. Theoret. Appl. Genetics 83, 963–967 (1992). https://doi.org/10.1007/BF00232957
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DOI: https://doi.org/10.1007/BF00232957