Abstract
A significant reduction in regeneration potential with increasing age (upto 12months) in rice (Oryza sativa L. cv.TN-1) embryogenic callus cultures was observed. Spermidine, while having an inhibitory effect on plant regeneration in fresh callus cultures, promoted morphogenesis in long-term callus cultures. A massive accumulation of polyamines, particularly putrescine (5-fold) was observed in 12 month old cultures resulting in a change of putrescine /spermidine ratio, which seems to be important for maintaining the morphogenetic response. Application of exogenous spermidine to 12 month old cultures showed increased levels of polyamines and restored the putrescine/spermidine ratio comparable to that found in freshly induced cultures, concomitantly, promoting the plant regeneration via somatic embryogenesis in long-term rice callus cultures.
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Abbreviations
- PA:
-
Polyamines
- PCA:
-
Perchloric acid
- PUT:
-
Putrescine
- SPD:
-
Spermidine
- SPM:
-
Spermine
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Bajaj, S., Rajam, M.V. Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant Cell Reports 14, 717–720 (1995). https://doi.org/10.1007/BF00232654
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DOI: https://doi.org/10.1007/BF00232654