Abstract
On a standard shoot culture medium, nodal cultures of Sarpagandha (Rauvolfia serpentina) could be maintained for nine months at 25° C by replacing cotton plugs with polypropylene caps as enclosures for culture tubes. Low temperature incubation of in vitro cultures appeared highly promising because cultures exhibited normal health even after 15 months of storage at 15° C; while 10°C and 5°C were found deleterious to growth of the cultures of R. serpentina.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anonymous (1969) In: The Wealth of India, Raw Materials, Vol VIII, Publications and Information Directorate, CSIR, New Delhi, India
Arora R, Bhojwani S S (1989) Plant Cell Reports 8: 44–47
Balachandran S M, Bhat S R, Chandel K P S (1990) Plant Cell Reports 8: 521–524
Bhojwani S S, Arumugam N, Arora R, Upadhyaya R P (1989) Indian J Plant Genet Resources 2: 103–113
Chaturvedi H C, Sharma M (1986) Proc 6th Intl Cong Plant Tissue Cell Cult, Univ Minnesota, Minneapolis, MN, p 429
El-Gizawy A M, Ford-Lloyd B V (1987) Plant Cell Tiss Org Cult 9: 147–150
Gupta R (1989) Indian J Plant Genet Resources 1: 98–102
Kartha K K (1985) Cryopreservation of Plant Cells and Organs, CRC Press, Boca Raton, FL
Mathur A, Mathur A K, Kukreja A K, Ahuja P S, Tyagi B R (1987) Plant Cell Tiss Org Cult 10: 129–134
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nayar MP, Sastry A R K (1987) Red Data Book of Indian Plants, Vol I, Botanical Survey of India, Calcutta, India
Wanas W H, Callow J A, Withers L A (1985) In: Withers L A, Alderson P G (Eds) Plant Tissue Culture and its Agricultural Applications, Butterworths, London, pp 285–290
Withers L A (1980) Int Board Plant Genet Resources Technical Report, IBPGR, Rome, p 91
Withers L A, Williams J T (1986) Int Board Plant Genet Resources Research Highlights, 1984–1985. IBPGR, FAO, Rome, p 21
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Rights and permissions
About this article
Cite this article
Sharma, N., Chandel, K.P.S. Low-temperature storage of Rauvolfia serpentina Benth. ex Kurz.: An endangered, endemic medicinal plant. Plant Cell Reports 11, 200–203 (1992). https://doi.org/10.1007/BF00232533
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232533