Summary
Marigold (Tagetes patula L.) hairy roots induced by infection with Agrobacterium rhizogenes produced α-terthienyl when grown in darkness, and an n-hexane extract of the roots showed nematocidal activity. Depending on the hairy root line used, the level of α-terthienyl varied from 15 to 1268 μg per g dry weight, a level that corresponded to 0.15 to 12.7-fold that in intact roots. Analysis by HPLC indicated that the nematocidal activity was due predominantly to α-terthienyl. However, it is suggested that nematocidal compounds other than α-terthienyl are present in hairy roots cultured in the dark for long periods or in the light.
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Communicated by F. Constabel
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Kyo, M., Miyauchi, Y., Fujimoto, T. et al. Production of nematocidal compounds by hairy root cultures of Tagetes patula L.. Plant Cell Reports 9, 393–397 (1990). https://doi.org/10.1007/BF00232407
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DOI: https://doi.org/10.1007/BF00232407