Abstract
Culture conditions for high frequency somatic embryogenesis and plant regeneration from cotyledonary explants of Codonopsis lanceolata are described. The maximum induction frequency of somatic embryos from cotyledonary explants was 80% on Murashige and Skoog (MS) medium containing 6% sucrose with 1 mg/l 2,4-dichlorophenoxyacetic acid and 10% coconut water. Upon transfer onto MS basal medium containing 3% sucrose, most somatic embryos developed into plantlets.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellin a3
- MS:
-
Murashige and Skoog
References
Ahn CS, Chung SJ, Koh GC, Park SH (1986) J Korean Soc Hort Sci 27:205–212
Harn C. Lee YI (1976) Korean J Plant Tissue Culture 4:1–3
Lee MS, Lee JH, Kim DY (1980) Korean J Plant Tissue Culture 7:9–12
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Sass JE (1971) Botanical microtechnique. 3rd ed., The Iowa State Univ. Press, Ames, Iowa, pp 55–77
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Communicated by I. K. Vasil
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Min, S.R., Yang, S.G., Liu, J.R. et al. High frequency somatic embryogenesis and plant regeneration in tissue cultures of Codonopsis lanceolata . Plant Cell Reports 10, 621–623 (1992). https://doi.org/10.1007/BF00232383
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DOI: https://doi.org/10.1007/BF00232383