Abstract
Fertile plants have been obtained from maize (Zea mays L.) embryogenic suspension culture protoplasts. Friable, embryogenic callus initiated from an immature embryo from a cross involving the genotypes A188, B73, and Black Mexican sweetcorn was used to establish a rapidly growing embryogenic suspension culture. After nine months in culture, high yields of viable protoplasts (30×106/ gram fresh weight) were obtained following a 1.5 hour enzymatic digestion. Protoplasts cultured with feeder cells divided and formed embryogenic callus, from which male and female fertile plants were regenerated. Protoplast-derived R1 plants were self-pollinated and immature R2 embryos isolated for callus initiation. Female fertile plants have also been produced from protoplasts isolated from an R2-derived suspension culture. Significant interactions between protoplast and feeder-cell lines were observed.
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Abbreviations
- BC:
-
backcross
- BMS:
-
Black Mexican Sweetcorn
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- PWS:
-
protoplast wash solution (0.2 M mannitol, 80 mM CaCl2)
- FDA:
-
fluorescein diacetate
- ABA:
-
abscisic acid
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Communicated by I. K. Vasil
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Petersen, W.L., Sulc, S. & Armstrong, C.L. Effect of nurse cultures on the production of macro-calli and fertile plants from maize embryogenic suspension culture protoplasts. Plant Cell Reports 10, 591–594 (1992). https://doi.org/10.1007/BF00232376
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DOI: https://doi.org/10.1007/BF00232376