Summary
In hippocampal pyramidal cells (HPCs), Dopamine (DA) application (1 μM) produced, in 50% of recorded cells, an hyperpolarization of the resting membrane potential (r.m.p.) and an increase of the afterhyperpolarization (AHP) amplitude and duration in 79% of recorded cells. DA-induced effects on both the r.m.p. and AHP were mimicked by bath application of a D-l selective agonist, SKF 38393 (20 μM). In addition, we have observed that a D-l selective antagonist such as SCH 23390 (1 μ,M) abolished the action of both DA and SKF 38393. In contrast, the activation of D-2 receptors through LY 171555 (10 μm) produced, in 50% of cells, a depolarization of the r.m.p. and a depression of the AHP in 67% of recorded cells. These results suggest that the effects observed in hippocampal pyramidal neurons after DA application of micromolar concentration are mediated by D-1 subtype of receptors.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alger BE, Nicoll RA (1980) Epileptiform burst afterhyperpolarization: calcium-dependent potassium potential in hippocampal CA1 pyramidal cells. Science 210:1122–1124
Benardo LS, Prince DA (1982a) Dopamine modulates a calciumactivated potassium conductance in mammalian hippocampal pyramidal cells. Nature 297:76–79
Benardo LS, Prince DA (1982b) Dopamine action on hippocampal pyramidal cells. J Neuroscience 2:415–423
Bischoff S, Scatton B, Korf J (1979) Biochemical evidence for a transmitter role of dopamine in the rat hippocampus. Brain Res 165:161–165
Bischoff S, Bittiger H, Kraus J (1980) “In vivo” [3H]spiperone binding to the rat hippocampal formation: involvement of dopamine receptors. Eur J Pharmacol 68:305–315
Biscoe TJ, Straughan DW (1966) Micro-electrophoretic studies of neurones in the cat hippocampus. J Physiol (Lond) 183:341–359
Calabresi P, Mercuri N, Stanzione P, Stefani A, Bernardi G (1987) Intracellular studies on the dopamine-induced firing inhibition of neostriatal neurons “in vitro”: evidence for D-1 receptor involvement. Neuroscience 20:757–771
Dawson TM, Gehlert DR, Yamamura HI, Barnett A, Wamsley JK (1985) D1 dopamine receptors in the rat brain: autoradiographic localization using [3H] SCH 23390. Eur J Pharmacol 108:323–325
Dinan TG, Crunelli V, Kelly JS (1987) Neuroleptics decrease calcium-activated potassium conductance in hippocampal pyramidal cells. Brain Res 407:159–162
Haas HL, Konnerth A (1983) Histamine and noradrenaline decrease calcium-activated potassium conductance in hippocampal pyramidal cells. Nature 302:432–434
Herrling PL (1981) The membrane potential of cat hippocampal neurons recorded “in vivo” displays four different reactionmechanisms to iontophoretically applied transmitter agonists. Brain Res 212:331–343
Hotson JR, Prince DA (1980) A calcium-activated hyperpolarization follows repetitive firing in hippocampal neurons. J Neurophysiol 43:409–419
Hotson JR, Prince DA, Schwartzkroin PA (1979) Anomalous inward rectification in hippocampal neurons. J Neurophysiol 42:889–895
Hyttel J (1983) SCH 23390: The first selective dopamine D-1 antagonist. Eur J Pharmacol 91:153–154
Madison DV, Nicoll RA (1984) Control of the repetitive discharge of rat CA1 pyramidal neurones “in vitro”. J Physiol (Lond) 354:319–331
Madison DV, Nicoll RA (1986a) Actions of noradrenaline recorded intracellularly in rat hippocampal CA1 pyramidal neurones. J Physiol (Lond) 372:221–244
Madison DV, Nicoll RA (1986b) Cyclic adenosine 3′,5′-monophosphate mediates β-receptor actions of noradrenaline in rat hippocampal pyramidal cells. J Physiol (Lond) 372:245–259
Malenka RC, Nicoll RA (1986) Dopamine decreases the calciumactivated afterhyperpolarization in hippocampal CA1 pyramidal cells. Brain Res 379:210–215
Pockett S (1985) Dopamine changes the shape of action potentials in hippocampal pyramidal cells. Brain Res 342:386–390
Scatton B, Dubois A (1985) Autoradiographic localization of D1 dopamine receptors in the rat brain with [3H] SKF 38393. Eur J Pharmacol 111:145–146
Scatton B, Simon H, Le Moal M, Bischoff S (1980) Origin of dopaminergic innervation of the rat hippocampal formation. Neurosci Lett 18:125–131
Schultz D, Stanford E, Wyrick S, Mailman R (1985) Binding of [3H] SCH 23390 in rat brain: regional distribution and effects of assay conditions and GTP suggest interaction at a D1 like dopamine receptor. J Neurochem 45:1601–1611
Schwartzkroin PA (1975) Characteristics of CA1 neurons recorded intracellularly in the hippocampal “in vitro” slice preparation. Brain Res 85:423–436
Schwartzkroin PA (1977) Further characteristics of hippocampal CA1 cells “in vitro”. Brain Res 128:53–68
Schwartzkroin PA, Slawsky M (1977) Probable calcium spikes in hippocampal neurons. Brain Res 135:157–161
Sibley DR, Leff SE, Creese I (1982) Interactions of novel dopaminergic ligands with D-1 and D-2 dopamine receptors. Life Sci 31:637–645
Simon H, Le Moal M, Calas A (1979) Efferents and afferents of the ventral tegmental-A 10 region studied after local injection of [3H] leucine and horseradish peroxidase. Brain Res 178:17–40
Smialowski A, Bijak M (1987) Excitatory and inhibitory action of dopamine on hippocampal neurons “in vitro”: involvement of D2 and D1 receptors. Neuroscience 23:95–101
Stanzione P, Calabresi P, Mercuri N, Bernardi G (1984) Dopamine modulates CA1 hippocampal neurons by elevating the threshold for spike generation: an “in vitro” study. Neuroscience 13:1105–1116
Stoof JC, Kebabian JW (1981) Opposing roles for D-1 and D-2 dopamine receptors in efflux of cyclic AMP from rat neostriatum. Nature 294:366–368
Suppes T, Kriegstein AR, Prince DA (1985) The influence of dopamine on epileptiform burst activity in hippocampal pyramidal neurons. Brain Res 326:273–280
Verney C, Baulack M, Berger B, Alvarez C, Vigny A, Helle K (1985) Morphological evidence for a dopaminergic terminal field in the hippocampal formation of young and adult rat. Neuroscience 14:1039–1052
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berretta, N., Berton, F., Bianchi, R. et al. Effects of dopamine, D-1 and D-2 dopaminergic agonists on the excitability of hippocampal CA1 pyramidal cells in guinea pig. Exp Brain Res 83, 124–130 (1990). https://doi.org/10.1007/BF00232200
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00232200