Abstract
An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10−3 M were transferred to hormone-free medium containing 10−2 M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10−4 M calcium were transferred to hormone-free medium with 10−3 M calcium. At calcium concentrations between 6·10−3 and 10−2 M globular stage somatic embryos were found in cultures supplemented with 2·10−6 M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
De Vries SC, Booij H, Janssens R, Vogels R, Saris L, LoSchiavo F, Terzi M, van Kammen A (1988a) Genes & Development 2:462–476
De Vries SC, Booij H, Meyerink P, Huisman G, Wilde HD, Thomas TL, van Kammen A (1988b) Planta 176:205–211
Ferguson IB, Drøbak BK (1988) HortScience 23:262–266
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:151–158
Halperin W, Jensen WA (1967) J Ultrastruct Res 18:428–443
Hepler PK (1988) In: Greuter W, Zimmer B (ed) Proc XIV Int Bot Congress, Koelz Scientific Books, Königstein, FRG, pp 225–240
Jarvis MC (1984) Plant Cell & Environm 7:153–164
Larkin PJ (1976) Planta 128:213–216
Mariani P, D'Orazi D, Bagni N (1989) J Plant Physiol 135:508–510
Nomura K (1987) PhD thesis, Univ Tokyo, Japan
Nomura K Komamine A (1986) Oxford Surveys Plant Mol Cell Biol 3:456–466
Penel C (1986) In: Greppin H, Penel C, Gaspar T (ed) Molecular and physiological aspects of plant peroxidases, University of Geneva, Switzerland, pp 155–164
Roberts DM Lukas TJ Watterson DM (1986) Crit Rev Plant Sci 4:311–339
Sung ZR (1976) Plant Physiol 57:460–462
Timmers ACJ De Vries SC Schel JHN (1989) Protoplasma 153:24–29
Author information
Authors and Affiliations
Additional information
Communicated by P. King
Rights and permissions
About this article
Cite this article
Jansen, M.A.K., Booij, H., Schel, J.H.N. et al. Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures. Plant Cell Reports 9, 221–223 (1990). https://doi.org/10.1007/BF00232184
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232184