Abstract
Callus was obtained from mericarps of Bunium persicum Boiss. on MS medium supplemented with 2.0 mg/1 2,4-D and 4.0 mg/1 Kn. Small white clumps of compactly packed cells developed on the callus on a medium containing 1.0 mg/1 2,4-D and 0 mg/1 Kn. These cell clumps differentiated numerous globular embryos on the same medium. Embryo maturation and germination was achieved on the basal as well as on 1 mg/1 Kn supplemented medium. All regenerated plants examined were normal diploids with 2n=14.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- Kn:
-
Kinetin
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Communicated by A. R. Gould
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Wakhlu, A.K., Nagari, S. & Barna, K.S. Somatic embryogenesis and plant regeneration from callus cultures of Bunium persicum Boiss. Plant Cell Reports 9, 137–138 (1990). https://doi.org/10.1007/BF00232089
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DOI: https://doi.org/10.1007/BF00232089