Abstract
Cotyledons excised from dark-grown seedlings of cucumber (Cucumis sativus L.) were cultured in vitro under UV radiation at different wavelengths, obtained by passage of light through cut-off filters with different transmittance properties. Growth and the synthesis of chlorophyll (Chl) in cotyledons were inhibited and malondialdehyde was accumulated upon irradiation at wavelengths below 320 nm. Exogenous application of scavengers of free radicals reversed the growth inhibition induced by UV-B. Measurement of the fluorescence of Chl a suggested that electron transfer in photosystems was affected by UV-B irradiation. On the basis of these results, the involvement is postulated of active species of oxygen in damages to thylakoid membranes and the growth inhibition that are induced by UV-B irradiation.
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Abbreviations
- Chl:
-
chlorophyll
- Fm :
-
maximal fluorescence (dark)
- F′m :
-
maximal fluorescence (light)
- Fv :
-
variable fluorescence (dark)
- F′v :
-
variable fluorescence (light)
- MDA:
-
malondialdehyde
- O2 :
-
Superoxide radical
- PS:
-
photosystem
- qN :
-
non-photochemical quenching of fluorescence
- qP :
-
photochemical quenching of fluorescence
- UV-BBE :
-
biologically effective UV-B radiation
- WL(T = 0.5):
-
wavelength at which 50% transmittance occurs
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Takeuchi, Y., Fukumoto, R., Kasahara, H. et al. Peroxidation of lipids and growth inhibition induced by UV-B irradiation. Plant Cell Reports 14, 566–570 (1995). https://doi.org/10.1007/BF00231939
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DOI: https://doi.org/10.1007/BF00231939