Summary
The projection from the intracerebellar nuclei to the paravermal (intermediate) cerebellar cortex of lobule Vb/c has been investigated in the cat using a combined electrophysiological and neuroanatomical technique. A small (10–30 nl) injection of WGA-HRP was made into one of the three paravermal zones (c1, c2 or c3) after the mediolateral boundaries of the zones had been delimited on the cerebellar surface by recording climbing fibre field potentials evoked in response to percutaneous stimulation of one or more paws. The distribution of retrogradely labelled cell bodies within the intracerebellar nuclei was compared with the distribution of terminal labelling arising from anterograde transport by cerebellar Purkinje cells. The three paravermal zones displayed marked heterogeneity in their receipt of a projection from the intracerebellar nuclei. The c1 and c3 zones received virtually no such input, although injections in either zone resulted in significant terminal labelling (which was largely restricted to nucleus interpositus anterior). By contrast, the intervening c2 zone received a much heavier nucleocortical input which arose almost exclusively from nucleus interpositus posterior (to which the zone also projected). A sparse contralateral nucleocortical input to the c2 zone was also demonstrated. This arose primarily from nucleus fastigius. It is concluded that the nucleocortical projection to the paravermal cortex of lobule Vb/c displays marked topographical specificity and some functional implications of this are discussed.
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References
Abercrombie M (1946) Estimation of nuclear population from microtome sections. Anat Record 94:239–247
Allen GI, Tsukuhara N, (1974) Cerebrocerebellar communication systems. Physiol Rev 54:957–1006
Angaut P, Buisseret-Delmas C, Compoint C (1988) Nucleocortical projections in the vermal A zone of the rat cerebellum: a retrograde and immunocytochemical study. Eur Neurosci Meeting, Abstr p 135
Batini C, Buisseret-Delmas C, Compoint C, Daniel H (1989) The GABAergic neurones of the cerebellar nuclei in the rat: projections to the cerebellar cortex. Neurosci Lett 99:251–256
Bishop GA, McCrea RA, Lightall JW, Kitai S (1979) An HRP and autoradiographic study of the projection from the cerebellar cortex to the nucleus interpositus anterior and nucleus interpositus posterior of the cat. J Comp Neurol 185:735–756
Brodal A, Kawamura K (1980) Olivocerebellar projection: a review. In: Brodal A et al (eds) Advances in anatomy, embryology and cell biology. Vol 64. Springer, Berlin Heidelberg New York Tokyo, pp 1–140
Buisseret-Delmas C, Angaut P (1988) The cerebellar nucleocortical projections in the rat: a retrograde labelling study using horseradish peroxidase combined to a lectin. Neurosci Lett 84:255–260
Chan-Palay V (1977) Cerebellar dentate nucleus, organization, cytology and transmitters. Springer, Berlin, pp 352–360
Chan-Palay V, Palay SL, Wu JY (1979) Gamma-aminobutyric acid pathways in the cerebellum studied by retrograde and anterograde transport of glutamic acid decarboxylase antibody after in vivo injections. Anat Embryol 157:1–14
Dietrichs E (1981) The cerebellar corticonuclear and nucleocortical projection in the cat as studied with anterograde and retrograde transport of horseradish peroxidase. III. The anterior lobe. Anat Embryol 162:223–247
Dietrichs E, Walberg F (1979) The cerebellar corticonuclear and nucleocortical projections in the cat as studied with anterograde and retrograde transport of HRP. I. Paramedian lobule. Anat Embryol 158:13–39
Dietrichs E, Walberg F (1980) The cerebellar corticonuclear and nucleocortical projections in the cat as studied with anterograde and retrograde transport of horseradish peroxidase. II. Lobulus simplex, crus I and II. Anat Embryol 161:83–103
Eccles JC, Ito M, Szentagothai J (1967) The cerebellum as a neuronal machine, Chap VII. Springer, Berlin Heidelberg New York
Ekerot CF, Larson B (1979) The dorsal spino-olivo-cerebellar system in the cat. I. Functional organization and termination in the anterior lobe. Exp Brain Res 36:201–217
Gould BB (1979) The organization of afferents to the cerebellar cortex in the cat: projections from the deep cerebellar nuclei. J Comp Neurol 184:27–42
Gould BB, Graybiel AM (1976) Afferents to the cerebellar cortex in the cat: evidence for an intrinsic pathway leading from the deep nuclei to the cortex. Brain Res 110:601–611
Haines DE (1978) Contralateral nucleocortical cells of the paraflocculus of tree shrew (Tupaia glis). Neurosci Lett 8:183–190
Haines DE (1989) An HRP study of cerebellar corticonuclear-nucleocortical topography of the dorsal culminate lobule-lobule V in a prosimian primate (Galago): with comments on nucleocortical cell types. J Comp Neurol 282:274–292
Haines DE, Patrick GW (1981) Cerebellar corticonuclear fibres of the paramedian lobule of tree shrew (Tupaia glis) with comments on zones. J Comp Neurol 201:99–119
Haines DE, Pearson JC (1979) Cerebellar corticonuclear-nucleocortical topography: a study of the tree shrew (Tupaia) paraflocculus. J Comp Neurol 187:745–758
Haines DE, Patrick GW, Satrulsee P (1982) Organization of cerebellar corticonuclear fiber systems. Exp Brain Res Ser 6:320–371
Haines DE, Rubertone JA (1979) Cerebellar corticonuclear fibers of the dorsal culminate lobule (anterior lobe-lobule V) in a prosimian primate, Galago senegalensis. J Comp Neurol 186:321–342
Hamori J, Mezey E, Szentagothai J (1981) Electron microscopic identification of cerebellar nucleo-cortical mossy terminals in the rat. Exp Brain Res 44:97–100
Hess DT (1982): Cerebellar nucleo-cortical neurons projecting to the vermis of lobule VII in the rat. Brain Res 248:361–366
Jansen J, Brodal A (1940) Experimental studies on the intrinsic fibres of the cerebellum. II. The corticonuclear projection. J Comp Neurol 73:267–321
Larson B, Miller S, Oscarsson O (1969) A spinocerebellar climbing fibre path activated by the flexor reflex afferents from all four limbs. J Physiol 203:641–649
Legendre A, Courville J (1986) Cerebellar nucleocortical projection with a survey of factors affecting the transport of radioactive tracers. J Comp Neurol 252:392–403
McCrea RA, Bishop GA, Kitai ST (1978) Morphological and electrophysiological characteristics of projection neurones in nucleus interpositus of the cat cerebellum. J Comp Neurol 181:397–420
Mesulam MM (1982) In: Tracing neural connections with horseradish peroxidase. IBRO Handbook, Wiley J, New York
Oscarsson O (1980) Functional organization of olivary projection to the cerebellar anterior lobe. In: Courville J et al (eds) The inferior olivary nucleus: anatomy and physiology. Raven, New York, pp 279–289
Palay SL, Chan-Palay V (1974) Cerebellar cortex: cytology and organization. Springer, Berlin Heidelberg New York
Palkovits M, Magyar P, Szentagothai J (1971) Quantitative histological analysis of the cerebellar cortex in the cat. I. Number and arrangement in space of the Purkinje cells. Brain Res 32:1–13
Payne JN (1983) The cerebellar nucleo-cortical projection in the rat studied by the retrograde fluorescent double-labelling method. Brain Res 271:141–144
Tolbert DL (1982) The cerebellar nucleocortical pathway. In: Palay SL, Chan-Palay V (eds) The cerebellum new vistas. Exp Brain Res Ser 6:296–319
Tolbert DL, Bantli H, Bloedel JR (1976) Anatomical and physiological evidence for a cerebellar nucleocortical projection in the cat. Neuroscience 1:205–217
Tolbert DL, Bantli H, Bloedel JR (1977) The intracerebellar nucleocortical projection in a primate. Exp Brain Res 30:425–434
Tolbert DL, Bantli H, Bloedel JR (1978a) Organizational features of the cat and monkey cerebellar nucleocortical projection. J Comp Neurol 182:39–56
Tolbert DL, Bantli H, Bloedel JR (1978b) Multiple branching of cerebellar efferent projections in cats. Exp Brain Res 31:305–316
Tolbert DL, Kultas-Ilinsky K, Ilinsky I (1980) EM autoradiography of cerebellar nucleocortical terminals in the cat. Anat Embryol 161:215–223
Trott JR, Armstrong DM (1987a) The cerebellar corticonuclear projection from lobule Vb/c of the cat anterior lobe: a combined electrophysiological and autoradiographic study. I. Projections from the intermediate region. Exp Brain Res 66:318–338
Trott JR, Armstrong DM (1987b) The cerebellar corticonuclear projection from lobule Vb/c of the cat anterior lobe: a combined electrophysiological and autoradiographic study. II. Projections from the vermis. Exp Brain Res 68:339–354
Trott JR, Armstrong DM (1987c) Olivocorticonuclear organisation within lobule V of the anterior lobe of the cat cerebellum. In: King JS (ed) New concepts in cerebellar neurobiology. Alan R Liss, New York, pp 221–238
Voogd J, Bigaré F (1980) The topographical distribution of olivary and corticonuclear fibers in the cerebellum: a review. In: Courville J, de Montigny C, Lamarre Y (eds) The inferior olivary nucleus: anatomy and physiology. Raven, New York, pp 207–234
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Trott, J.R., Apps, R. & Armstrong, D.M. Topographical organisation within the cerebellar nucleocortical projection to the paravermal cortex of lobule Vb/c in the cat. Exp Brain Res 80, 415–428 (1990). https://doi.org/10.1007/BF00228169
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DOI: https://doi.org/10.1007/BF00228169