Summary
The number of identical S-alleles between two wild populations of B. campestris, one in Turkey, the other in Japan, that have been independent of one another for a long time was investigated. Diallel pollination tests between 38 S-allele homozygotes, i.e., 16 S-allele homozygotes from Turkey and 22 from Japan, revealed that these were 29 different S-alleles only 4 common ones. These S-alleles were differentiated by the iso-electric focusing (IEF) analysis of S-locus glycoproteins (SLGs) stained with an antiserum against SLG8. All identical S-alleles had the major SLG band at the same pI value without exception, even though they were collected from different populations. However, the number of minor bands of SLGs varied between the two populations; the S-alleles in Balcesme had generally fewer minor bands than those in Oguni. The 29 independent S-alleles were numbered from S 21 to S 49 according to the pI value of the major SLG band. The major bands whose pI values were 7.5–8.5 were most common. Blot-hybridization patterns of genomic DNA hybridized with SLG 8 cDNA were not always the same among the strains of identical S-alleles obtained from different populations. Because about 20% of the S-alleles were shared between the two populations, it can be inferred that more than hundreds of S-alleles have been accumulated by mutation in B. campestris throughout the world.
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References
Bateman AJ (1955) Self-incompatibility systems in angiosperms. III. Cruciferae. Heredity 9:52–68
Chen CH, Nasrallah JB (1990) A new class of S sequences defined by a pollen recessive self-incompatibility allele of Brassica olevacea. Mol Gen Genet 222:241–248
Dwyer KG, Balnet MA, Nasrallah JB, Nasrallah ME (1991) DNA sequences of self-incompatibility genes from Brassica campestris and B. oleracea: polymorphism predating speciation. Plant Mol Biol 16:481–486
Gaude T, Denoroy L, Dumas C (1991) Use of a fast protein electrophoretic purification procedure for N-terminal sequence analysis to identify S-locus-related proteins in stigmas of Brassica oleracea. Electrophoresis 12:646–653
Hinata K, Watanabe M, Toriyama K, Isogai A (1993) A review of recent studies on homomorphic self-incompatibility. Int Rev Cytol 143:257–296
Isogai A, Takayama S, Tsukamoto C, Ueda Y, Shiozawa H, Hinata K, Okazaki K, Suzuki A (1987) S-locus-specific glycoproteins associated with self-incompatibility in Brassica campestris. Plant Cell Pyhsiol 28:1279–1291
Isogai A, Yamagawa S, Watanabe M, Hinata K, Suzuki A (1991) S- and NS-glycoproteins of Brassica campestris. In: Third Int Congr Plant Mol Biol molecular biology of plant growth and development. Abstr no. 526
Lalonde BA, Nasrallah ME, Dwyer KG, Chen CH, Barlow B, Nasrallah JB (1989) A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene. Plant Cell 1:249–258
Nasrallah JB, Kao TH, Goldberg ML, Nasrallah ME (1987) Amino acid sequence of glycoproteins encoded by three alleles of the S-locus of Brassica oleracea. Nature 326:617–619
Nou IS, Watanabe M, Isogai A, Shiozawa H, Suzuki A, Hinata K (1991) Variation of S-alleles and S-glycoproteins in a naturalized population of self-incompatible Brassica campestris L. Jpn J Genet 66:227–239
Nou IS, Watanabe M, Isuzugawa K, Isogai A, Hinata K (1993) Isolation of S-alleles from a wild population of Brassica campestris L. at Balcesme, Turkey and their characterization by S-glycoproteins. Sex Plant Reprod 6:71–78
Ockendon DJ (1974) Distribution of self-incompatibility alleles and breeding structure of open-pollinated cultivars of Brussels sprouts. Heredity 33:159–171
Ockendon DJ (1982) An S-allele survey of cabbage (Brassica oleracea var ‘capitata’). Euphytica 31:325–331
Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69
Stein JC, Howlett B, Boyes DC, Nasrallah ME, Nasrallah JB (1991) Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci USA 88:8816–8820
Takayama S, Isogai A, Tsukamoto C, Ueda Y, Hinata K, Okazaki K, Suzuki A (1986a) Isolation and some characterization of S-locus-specific glycoproteins associated with self-incompatibility in Brassica campestris. Agric Biol Chem 50:1365–1367
Takayama S, Isogai A, Tsukamoto C, Ueda Y, Hinata K, Okazaki K, Koseki K, Suzuki A (1986b) Structure of carbohydrate chains of S-glycoproteins in Brassica campestris associated with self-incompatibility. Agric Biol Chem 50:1673–1676
Takayama S, Isogai A, Tsukamoto C, Ueda Y, Hinata K, Okazaki K, Suzuki A (1987) Sequences of S-glycoproteins, products of the Brassica campestris self-incompatibility locus. Nature 326:102–105
Trick M, Flavell RB (1989) A homologous S genotype of Brassica oleracea expresses two S-like genes. Mol Gen Genet 218:112–117
Umbach AL, Lalonde BA, Kandasamy MK, Nasrallah JB, Nasrallah ME (1990) Immunodetection of protein glycoforms encoded by two independent genes of the self-incompatibility multigene family of Brassica. Plant Physiol 93:739–747
Watanabe M, Nou IS, Takayama S, Yamakawa S, Isogai A, Suzuki A, Takeuchi T, Hinata K (1992) Variations in and inheritance of NS-glycoprotein in self-incompatible Brassica campestris L. Plant Cell Physiol 33:343–351
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Nou, S., Watanabe, M., Isogai, A. et al. Comparison of S-alleles and S-glycoproteins between two wild populations of Brassica campestris in Turkey and Japan. Sexual Plant Reprod 6, 79–86 (1993). https://doi.org/10.1007/BF00227652
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DOI: https://doi.org/10.1007/BF00227652